A simplified coculture system with luteinized granulosa cells improves embryo quality and implantation rates: A controlled study
To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET. Controlled clinical study. IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center. Patients with poor embryo quality in their previous IVF-ET cyc...
Gespeichert in:
| Veröffentlicht in: | Fertility and sterility 1997, Vol.67 (1), p.120-122 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 122 |
|---|---|
| container_issue | 1 |
| container_start_page | 120 |
| container_title | Fertility and sterility |
| container_volume | 67 |
| creator | Dirnfeld, Martha Goldman, Shlomit Gonen, Yael Koifman, Mara Calderon, Ilan Abramovici, Haim |
| description | To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET.
Controlled clinical study.
IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center.
Patients with poor embryo quality in their previous IVF-ET cycles.
Embryos from 40 patients, in which >50% of the embryos were classified as poor quality in their previous IVF attempts, were grown on autologue GC culture system for a short period (24–48 hours) before being replaced in the uterine cavity.
Embryo quality.
Significant decrease in poor quality embryos and increase in the proportion of good quality embryos were observed using a coculture system with autologue human GCs. Pregnancy rates in this groups of patients reached our standard IVF results during the same period.
This study describes a simplified short-term coculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly. |
| doi_str_mv | 10.1016/S0015-0282(97)81867-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78776669</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0015028297818675</els_id><sourcerecordid>78776669</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-a27c26961a9d03dba37af193b958993b05cd05fde9a060cbbd08fcd54fb5ca5e3</originalsourceid><addsrcrecordid>eNqFkEtv1DAURi0EKkPhJ1TyAqGyCNhJ7dhs0KjiJVXqAlhbjn0DRk7c-jpF6ao_HU9nNFtWd_Gd-zqEnHH2jjMu339njIuGtao91_1bxZXsG_GEbLgQshFSdE_J5og8Jy8Q_zDGJO_bE3KitJJSiw152FIM000MYwBPXXJLLEsGiisWmOjfUH7TuBQIc7ivwK9s5yUmtNRBjEhra053gBSmIa-J3i42hrJSO_tdFu1cbAlpptkWwA90W1fMJacY6zAsi19fkmejjQivDvWU_Pz86cfl1-bq-su3y-1V4y46WRrb9q6VWnKrPev8YLvejlx3gxZK18KE80yMHrRlkrlh8EyNzouLcRDOCuhOyZv93Hrw7QJYzBRw94SdIS1oetX3sjqpoNiDLifEDKO5yWGyeTWcmZ1582je7LQa3ZtH80bUvrPDgmWYwB-7Dqpr_vqQW3Q2jtWkC3jEWtFKqbqKfdxjUGXcBcgGXYDZgQ8ZXDE-hf8c8g_MFKQC</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78776669</pqid></control><display><type>article</type><title>A simplified coculture system with luteinized granulosa cells improves embryo quality and implantation rates: A controlled study</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>ScienceDirect Journals (5 years ago - present)</source><source>Alma/SFX Local Collection</source><creator>Dirnfeld, Martha ; Goldman, Shlomit ; Gonen, Yael ; Koifman, Mara ; Calderon, Ilan ; Abramovici, Haim</creator><creatorcontrib>Dirnfeld, Martha ; Goldman, Shlomit ; Gonen, Yael ; Koifman, Mara ; Calderon, Ilan ; Abramovici, Haim</creatorcontrib><description>To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET.
Controlled clinical study.
IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center.
Patients with poor embryo quality in their previous IVF-ET cycles.
Embryos from 40 patients, in which >50% of the embryos were classified as poor quality in their previous IVF attempts, were grown on autologue GC culture system for a short period (24–48 hours) before being replaced in the uterine cavity.
Embryo quality.
Significant decrease in poor quality embryos and increase in the proportion of good quality embryos were observed using a coculture system with autologue human GCs. Pregnancy rates in this groups of patients reached our standard IVF results during the same period.
This study describes a simplified short-term coculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(97)81867-5</identifier><identifier>PMID: 8986695</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Birth control ; Cells, Cultured ; coculture effect ; Coculture Techniques ; embryo culture in vitro ; embryo quality ; Embryo Transfer ; Failed IVF-ET ; Female ; Fertilization in Vitro ; granulosa cells ; Granulosa Cells - physiology ; Gynecology. Andrology. Obstetrics ; Humans ; Medical sciences ; Organ Culture Techniques ; Pregnancy ; Sterility. Assisted procreation</subject><ispartof>Fertility and sterility, 1997, Vol.67 (1), p.120-122</ispartof><rights>1997 American Society for Reproductive Medicine</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-a27c26961a9d03dba37af193b958993b05cd05fde9a060cbbd08fcd54fb5ca5e3</citedby><cites>FETCH-LOGICAL-c436t-a27c26961a9d03dba37af193b958993b05cd05fde9a060cbbd08fcd54fb5ca5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0015-0282(97)81867-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,4012,27910,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2526683$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8986695$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dirnfeld, Martha</creatorcontrib><creatorcontrib>Goldman, Shlomit</creatorcontrib><creatorcontrib>Gonen, Yael</creatorcontrib><creatorcontrib>Koifman, Mara</creatorcontrib><creatorcontrib>Calderon, Ilan</creatorcontrib><creatorcontrib>Abramovici, Haim</creatorcontrib><title>A simplified coculture system with luteinized granulosa cells improves embryo quality and implantation rates: A controlled study</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET.
Controlled clinical study.
IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center.
Patients with poor embryo quality in their previous IVF-ET cycles.
Embryos from 40 patients, in which >50% of the embryos were classified as poor quality in their previous IVF attempts, were grown on autologue GC culture system for a short period (24–48 hours) before being replaced in the uterine cavity.
Embryo quality.
Significant decrease in poor quality embryos and increase in the proportion of good quality embryos were observed using a coculture system with autologue human GCs. Pregnancy rates in this groups of patients reached our standard IVF results during the same period.
This study describes a simplified short-term coculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly.</description><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cells, Cultured</subject><subject>coculture effect</subject><subject>Coculture Techniques</subject><subject>embryo culture in vitro</subject><subject>embryo quality</subject><subject>Embryo Transfer</subject><subject>Failed IVF-ET</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>granulosa cells</subject><subject>Granulosa Cells - physiology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Organ Culture Techniques</subject><subject>Pregnancy</subject><subject>Sterility. Assisted procreation</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAURi0EKkPhJ1TyAqGyCNhJ7dhs0KjiJVXqAlhbjn0DRk7c-jpF6ao_HU9nNFtWd_Gd-zqEnHH2jjMu339njIuGtao91_1bxZXsG_GEbLgQshFSdE_J5og8Jy8Q_zDGJO_bE3KitJJSiw152FIM000MYwBPXXJLLEsGiisWmOjfUH7TuBQIc7ivwK9s5yUmtNRBjEhra053gBSmIa-J3i42hrJSO_tdFu1cbAlpptkWwA90W1fMJacY6zAsi19fkmejjQivDvWU_Pz86cfl1-bq-su3y-1V4y46WRrb9q6VWnKrPev8YLvejlx3gxZK18KE80yMHrRlkrlh8EyNzouLcRDOCuhOyZv93Hrw7QJYzBRw94SdIS1oetX3sjqpoNiDLifEDKO5yWGyeTWcmZ1582je7LQa3ZtH80bUvrPDgmWYwB-7Dqpr_vqQW3Q2jtWkC3jEWtFKqbqKfdxjUGXcBcgGXYDZgQ8ZXDE-hf8c8g_MFKQC</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Dirnfeld, Martha</creator><creator>Goldman, Shlomit</creator><creator>Gonen, Yael</creator><creator>Koifman, Mara</creator><creator>Calderon, Ilan</creator><creator>Abramovici, Haim</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1997</creationdate><title>A simplified coculture system with luteinized granulosa cells improves embryo quality and implantation rates: A controlled study</title><author>Dirnfeld, Martha ; Goldman, Shlomit ; Gonen, Yael ; Koifman, Mara ; Calderon, Ilan ; Abramovici, Haim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-a27c26961a9d03dba37af193b958993b05cd05fde9a060cbbd08fcd54fb5ca5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Cells, Cultured</topic><topic>coculture effect</topic><topic>Coculture Techniques</topic><topic>embryo culture in vitro</topic><topic>embryo quality</topic><topic>Embryo Transfer</topic><topic>Failed IVF-ET</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>granulosa cells</topic><topic>Granulosa Cells - physiology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Organ Culture Techniques</topic><topic>Pregnancy</topic><topic>Sterility. Assisted procreation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dirnfeld, Martha</creatorcontrib><creatorcontrib>Goldman, Shlomit</creatorcontrib><creatorcontrib>Gonen, Yael</creatorcontrib><creatorcontrib>Koifman, Mara</creatorcontrib><creatorcontrib>Calderon, Ilan</creatorcontrib><creatorcontrib>Abramovici, Haim</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dirnfeld, Martha</au><au>Goldman, Shlomit</au><au>Gonen, Yael</au><au>Koifman, Mara</au><au>Calderon, Ilan</au><au>Abramovici, Haim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simplified coculture system with luteinized granulosa cells improves embryo quality and implantation rates: A controlled study</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>1997</date><risdate>1997</risdate><volume>67</volume><issue>1</issue><spage>120</spage><epage>122</epage><pages>120-122</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET.
Controlled clinical study.
IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center.
Patients with poor embryo quality in their previous IVF-ET cycles.
Embryos from 40 patients, in which >50% of the embryos were classified as poor quality in their previous IVF attempts, were grown on autologue GC culture system for a short period (24–48 hours) before being replaced in the uterine cavity.
Embryo quality.
Significant decrease in poor quality embryos and increase in the proportion of good quality embryos were observed using a coculture system with autologue human GCs. Pregnancy rates in this groups of patients reached our standard IVF results during the same period.
This study describes a simplified short-term coculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>8986695</pmid><doi>10.1016/S0015-0282(97)81867-5</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0015-0282 |
| ispartof | Fertility and sterility, 1997, Vol.67 (1), p.120-122 |
| issn | 0015-0282 1556-5653 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78776669 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present); Alma/SFX Local Collection |
| subjects | Biological and medical sciences Birth control Cells, Cultured coculture effect Coculture Techniques embryo culture in vitro embryo quality Embryo Transfer Failed IVF-ET Female Fertilization in Vitro granulosa cells Granulosa Cells - physiology Gynecology. Andrology. Obstetrics Humans Medical sciences Organ Culture Techniques Pregnancy Sterility. Assisted procreation |
| title | A simplified coculture system with luteinized granulosa cells improves embryo quality and implantation rates: A controlled study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T11%3A00%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20simplified%20coculture%20system%20with%20luteinized%20granulosa%20cells%20improves%20embryo%20quality%20and%20implantation%20rates:%20A%20controlled%20study&rft.jtitle=Fertility%20and%20sterility&rft.au=Dirnfeld,%20Martha&rft.date=1997&rft.volume=67&rft.issue=1&rft.spage=120&rft.epage=122&rft.pages=120-122&rft.issn=0015-0282&rft.eissn=1556-5653&rft.coden=FESTAS&rft_id=info:doi/10.1016/S0015-0282(97)81867-5&rft_dat=%3Cproquest_cross%3E78776669%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78776669&rft_id=info:pmid/8986695&rft_els_id=S0015028297818675&rfr_iscdi=true |