A simplified coculture system with luteinized granulosa cells improves embryo quality and implantation rates: A controlled study

To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET. Controlled clinical study. IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center. Patients with poor embryo quality in their previous IVF-ET cycles. Embryos from 40 patients, in which >50% of the embryos were classified as poor quality in their previous IVF attempts, were grown on autologue GC culture system for a short period (24–48 hours) before being replaced in the uterine cavity. Embryo quality. Significant decrease in poor quality embryos and increase in the proportion of good quality embryos were observed using a coculture system with autologue human GCs. Pregnancy rates in this groups of patients reached our standard IVF results during the same period. This study describes a simplified short-term coculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly.