Crystallization and preliminary X-ray studies of mouse tumor necrosis factor

Crystallization and preliminary X-ray studies of mouse tumor necrosis factor

Well diffracting crystals of recombinant mouse tumor necrosis factor (mTNF) have been obtained. The sitting‐drop vapor‐diffusion method was used to grow crystals suitable for X‐ray studies from solutions containing methoxypolyethylene glycol 2000 and isopropanol as precipitants. The crystals belong...

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Veröffentlicht in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 1997-05, Vol.53 (3), p.329-330
Hauptverfasser: Baeyens, K. J., De Bondt, H. L., Raeymaekers, A., De Ranter, C. J., Fiers, W.
Format: Artikel
Sprache:eng
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Zusammenfassung:Well diffracting crystals of recombinant mouse tumor necrosis factor (mTNF) have been obtained. The sitting‐drop vapor‐diffusion method was used to grow crystals suitable for X‐ray studies from solutions containing methoxypolyethylene glycol 2000 and isopropanol as precipitants. The crystals belong to the space group P1 with unit‐cell dimensions a = 49.40, b = 48.24, c = 51.13 Å, α = 115.06, β = 103.32, γ = 91.27° and one trimer in the asymmetric unit. Crystals are stable to X‐rays and diffract beyond 2.0 Å. Cooling the crystal during data collection is necessary since crystals dissolve at room temperature.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S090744499601534X