Improved removal of viruslike particles from purified monoclonal antibody IgM preparation via virus filtration

More and more, monoclonal antibodies (Mabs) are being used for in vivo applications. In immunological research, they are used to examine finely tuned immunological interactions in animal experiments. For example, monoclonal antibodies directed against TNF- alpha , lipid A, and lipopolysaccharide are used to study the rheumatic disease systemic lupus erythematodes in mice. As has been shown previously, hybridomas used in Mab production are often infected by rodent viruses and such infections, resulting from mab preparation contamination, can lead to contradictory interpretations of the resulting immune response.