Detection of tubulin-binding proteins by an overlay assay

A blot overlay technique to detect tubulin-binding proteins has been developed. It involves the fractionation of the putative tubulin-binding proteins by gel electrophoresis, their transfer to nitrocellulose paper, and the incubation of the nitrocellulose sheet with purified tubulin. The proteins wh...

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Veröffentlicht in:Analytical biochemistry 1988-11, Vol.175 (1), p.91-95
Hauptverfasser: Kremer, Leonor, Domínguez, Jorge E., Avila, Jesús
Format: Artikel
Sprache:eng
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Zusammenfassung:A blot overlay technique to detect tubulin-binding proteins has been developed. It involves the fractionation of the putative tubulin-binding proteins by gel electrophoresis, their transfer to nitrocellulose paper, and the incubation of the nitrocellulose sheet with purified tubulin. The proteins which bind tubulin are recognized by monoclonal anti-tubulin antibodies. Proteins which associate with microtubules through a direct interaction with tubulin are easily detected by this procedure, which has been tested for both known microtubule-associated proteins and newly reported tubulin-binding proteins.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(88)90365-X