Conversion of the Beckman liquid phase sequencer to a gas-liquid phase sequencer
As an effective aid to extend the microsequencing capabilities the Beckman protein/peptide sequenator Series 890C has been successfully converted to a gas-liquid system, in which coupling buffer 25% trimethylamine was employed as a gas, and heptafluorobutyric acid as a liquid. The system has been fo...
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| Veröffentlicht in: | Analytical biochemistry 1988-11, Vol.175 (1), p.39-51 |
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| container_end_page | 51 |
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| container_title | Analytical biochemistry |
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| creator | Bhown, Ajit S. Wayland, James L. Lynn, J.Daniel Bennett, J.Claude |
| description | As an effective aid to extend the microsequencing capabilities the Beckman protein/peptide sequenator Series 890C has been successfully converted to a gas-liquid system, in which coupling buffer 25% trimethylamine was employed as a gas, and heptafluorobutyric acid as a liquid. The system has been found to be efficient for microsequencing (< 100 pmol). The details of mechanical, plumbing, and other minor changes are described in this paper along with the results of sequencing proteins and peptides, directly and from blots. |
| doi_str_mv | 10.1016/0003-2697(88)90358-2 |
| format | Article |
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The system has been found to be efficient for microsequencing (< 100 pmol). 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The system has been found to be efficient for microsequencing (< 100 pmol). The details of mechanical, plumbing, and other minor changes are described in this paper along with the results of sequencing proteins and peptides, directly and from blots.</description><subject>Amino Acid Sequence</subject><subject>Chemistry Techniques, Analytical - instrumentation</subject><subject>Chromatography, Gas - instrumentation</subject><subject>Fluorocarbons</subject><subject>Indicators and Reagents</subject><subject>Methylamines</subject><subject>peptides</subject><subject>Solvents</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKAzEUhoMotVbfQCEr0cVo0lxnI2jxBgVd6DpkkjM22s7UZKbg2ztjS1eiq7P4b4cPoWNKLiih8pIQwrKxzNWZ1uc5YUJn4x00pCSXGWEk30XDrWUfHaT0TgilXMgBGrAxF0KpIXqe1NUKYgp1hesSNzPAN-A-FrbC8_DZBo-XM5sAJ_hsoXIQcVNji99syn7XD9FeaecJjjZ3hF7vbl8mD9n06f5xcj3NHNO6yRQrRAGWQClL6S0UnHQf5ZT5QmvlPedaEqGEZx6c88xacIVVRVFyJXku2QidrnuXse6mU2MWITmYz20FdZuM0koIzsW_RiooY4SNOyNfG12sU4pQmmUMCxu_DCWmJ256nKbHabQ2P8RNHzvZ9LfFAvw2tEHc6VdrHToaqwDRJBd6VD5EcI3xdfh74Bub4pBc</recordid><startdate>19881115</startdate><enddate>19881115</enddate><creator>Bhown, Ajit S.</creator><creator>Wayland, James L.</creator><creator>Lynn, J.Daniel</creator><creator>Bennett, J.Claude</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19881115</creationdate><title>Conversion of the Beckman liquid phase sequencer to a gas-liquid phase sequencer</title><author>Bhown, Ajit S. ; Wayland, James L. ; Lynn, J.Daniel ; Bennett, J.Claude</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-73b5bea0ef6f6daeb40455913db887dd44860575d3deccd3aaecba7bbf4764963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Amino Acid Sequence</topic><topic>Chemistry Techniques, Analytical - instrumentation</topic><topic>Chromatography, Gas - instrumentation</topic><topic>Fluorocarbons</topic><topic>Indicators and Reagents</topic><topic>Methylamines</topic><topic>peptides</topic><topic>Solvents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhown, Ajit S.</creatorcontrib><creatorcontrib>Wayland, James L.</creatorcontrib><creatorcontrib>Lynn, J.Daniel</creatorcontrib><creatorcontrib>Bennett, J.Claude</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhown, Ajit S.</au><au>Wayland, James L.</au><au>Lynn, J.Daniel</au><au>Bennett, J.Claude</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conversion of the Beckman liquid phase sequencer to a gas-liquid phase sequencer</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1988-11-15</date><risdate>1988</risdate><volume>175</volume><issue>1</issue><spage>39</spage><epage>51</epage><pages>39-51</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>As an effective aid to extend the microsequencing capabilities the Beckman protein/peptide sequenator Series 890C has been successfully converted to a gas-liquid system, in which coupling buffer 25% trimethylamine was employed as a gas, and heptafluorobutyric acid as a liquid. 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| ispartof | Analytical biochemistry, 1988-11, Vol.175 (1), p.39-51 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Chemistry Techniques, Analytical - instrumentation Chromatography, Gas - instrumentation Fluorocarbons Indicators and Reagents Methylamines peptides Solvents |
| title | Conversion of the Beckman liquid phase sequencer to a gas-liquid phase sequencer |
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