Conversion of the Beckman liquid phase sequencer to a gas-liquid phase sequencer

As an effective aid to extend the microsequencing capabilities the Beckman protein/peptide sequenator Series 890C has been successfully converted to a gas-liquid system, in which coupling buffer 25% trimethylamine was employed as a gas, and heptafluorobutyric acid as a liquid. The system has been found to be efficient for microsequencing (< 100 pmol). The details of mechanical, plumbing, and other minor changes are described in this paper along with the results of sequencing proteins and peptides, directly and from blots.