Biological activity of recombinant human ZP3 produced in vitro: potential for a sperm function test

The human zona binding test is the most predictive test of sperm function yet the availability of human zona severely restricts its clinical use. The primary aim of this study was to use a commercially available in-vitro transcription and translation system to produce immobilized recombinant human Z...

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Veröffentlicht in:	Molecular human reproduction 1996-12, Vol.2 (12), p.911-919
Hauptverfasser:	Whitmarsh, A.J., Woolnough, M.J., Moore, H.D.M., Hornby, D.P., Barratt, C.L.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrosome - physiology acrosome reaction Base Sequence DNA, Complementary - genetics Egg Proteins - biosynthesis Egg Proteins - genetics Egg Proteins - metabolism Female Humans In Vitro Techniques Male Membrane Glycoproteins - biosynthesis Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Plasmids - genetics Protein Biosynthesis Receptors, Cell Surface Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - metabolism spermatozoa Spermatozoa - physiology Transcription, Genetic zona zona binding Zona Pellucida Glycoproteins ZP3
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Zusammenfassung:	The human zona binding test is the most predictive test of sperm function yet the availability of human zona severely restricts its clinical use. The primary aim of this study was to use a commercially available in-vitro transcription and translation system to produce immobilized recombinant human ZP3 (rhuZP3) on agarose beads. The biological activity of this preparation was examined using sperm binding and the acrosome reaction. Significantly higher levels of sperm binding to rhuZP3 beads (n = 12, P
ISSN:	1360-9947 1460-2407
DOI:	10.1093/molehr/2.12.911