A New Trisaccharide Sugar Chain Linked to a Serine Residue in Bovine Blood Coagulation Factors VII and IX

A new trisaccharide sugar chain was identified in bovine blood coagulation factors VII and IX. A pentapeptide isolated from factor VII contained Ser-52, which could not be identified with a gas-phase sequencer, suggesting an unknown substituent on the serine residue (Takeya, H. et al. (1988) J. Biol...

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Veröffentlicht in:Journal of biochemistry (Tokyo) 1988, Vol.104 (6), p.867-868
Hauptverfasser: Hase, Sumihiro, Kawabata, Shun-ichiro, Nishimura, Hitoshi, Takeya, Hiroyuki, Sueyoshi, Tatsuya, Miyata, Toshiyuki, Iwanaga, Sadaaki, Takao, Toshifumi, Shimonishi, Yasutsugu, Ikenaka, Tokuji
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Sprache:eng
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Zusammenfassung:A new trisaccharide sugar chain was identified in bovine blood coagulation factors VII and IX. A pentapeptide isolated from factor VII contained Ser-52, which could not be identified with a gas-phase sequencer, suggesting an unknown substituent on the serine residue (Takeya, H. et al. (1988) J. Biol. Chem., in press). The same results were obtained for a pentapeptide containing Ser-53 of factor IX. Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose. This sugar component was also confirmed by high-resolution fast atom bombardment mass spectrometric analysis of the pentapeptide. The trisaccharide was released from the peptides by means of β-elimination reaction and its reducing end was coupled with 2-aminopyridine. The fluorescent pyridylamino (PA-) derivative of the trisaccharide was purified by gel-filtration and reversed-phase HPLC. The sugar composition of the PA-trisaccharide was found to be 2 mol of xylose and 1 mol of PA-glucose. These results indicate the existence of a (Xyl2)Glc-Ser structure in factors VII and IX.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a122571