Urinary catecholamines: Comparison between HPLC with electrochemical detection and fluorophotometric assay

Comparisons between catecholamines determined by HPLC (with electrochemical detection) and fluorometry in urine samples from healthy adults and children, respectively, showed high correlations. In agreement with greater specificity for HPLC, mean values were higher for the fluorometric assay. Howeve...

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Veröffentlicht in:	Pharmacology, biochemistry and behavior biochemistry and behavior, 1988-10, Vol.31 (2), p.287-289
Hauptverfasser:	Lundberg, Ulf, Holmberg, Lars, Frankenhaeuser, Marianne
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Age Factors Analytical, structural and metabolic biochemistry Biological and medical sciences Child, Preschool Chromatography, High Pressure Liquid - methods Electrochemistry Epinephrine - urine Female Fluorometry - methods Fluorophotometric assay Fundamental and applied biological sciences. Psychology HPLC Human studies Humans Male Middle Aged Non peptidic neurotransmitters, polyamines Norepinephrine - urine Other biological molecules Urinary catecholamines
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Zusammenfassung:	Comparisons between catecholamines determined by HPLC (with electrochemical detection) and fluorometry in urine samples from healthy adults and children, respectively, showed high correlations. In agreement with greater specificity for HPLC, mean values were higher for the fluorometric assay. However, it was concluded that fluorometric assays provide as valid and sensitive indices of stress-induced changes in catecholamine excretion in humans as HPLC.
ISSN:	0091-3057 1873-5177
DOI:	10.1016/0091-3057(88)90347-4