Glycation of H1-Histone

Glycation of H1-Histone

H1-histone (H1) purified from calf thymus was subejcted to nonenzymatic glycation in vitro. A lysine residue in a restricted environment, with an apparent pKa of 6.9, was glycated, notwithstanding the high lysine content of H1. The extent of glycation per lysine residue was 2.4 times larger than tha...

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Veröffentlicht in:Chemical & pharmaceutical bulletin 1988/07/25, Vol.36(7), pp.2716-2719
Hauptverfasser: SAKURAI, TAMIKO, TUCHIYA, SEISHI
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cattle
Chemical Phenomena
Chemistry
fluorescence
Fundamental and applied biological sciences. Psychology
Glucose
Histones - analysis
Proteins
Spectrophotometry, Ultraviolet
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Beschreibung
Zusammenfassung:H1-histone (H1) purified from calf thymus was subejcted to nonenzymatic glycation in vitro. A lysine residue in a restricted environment, with an apparent pKa of 6.9, was glycated, notwithstanding the high lysine content of H1. The extent of glycation per lysine residue was 2.4 times larger than that of human serum albumin. Fluorescence at Em. 420 nm (Ex. 340 nm) was closely correlated to the degree of glycation.
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.36.2716