Effect of mechanical pressure on c-fos and on the mitotic activity of epidermal cells

A dosed mechanical pressure of 12.5 kg/cm2 applied for 1 min on depilated mouse skin did not cause cellular death or visible alterations of the cellular ultrastructures. However, it had a strong effect on the mitotic cycle of the epidermal cells-stimulating the cells to enter the mitotic cycle and t...

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Veröffentlicht in:Acta biochimica polonica 1996-01, Vol.43 (4), p.593-601
Hauptverfasser: Tsanev, R, Yaneva, J, Vaptzarova, K, Markov, D
Format: Artikel
Sprache:eng
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Zusammenfassung:A dosed mechanical pressure of 12.5 kg/cm2 applied for 1 min on depilated mouse skin did not cause cellular death or visible alterations of the cellular ultrastructures. However, it had a strong effect on the mitotic cycle of the epidermal cells-stimulating the cells to enter the mitotic cycle and temporarily blocking the G1-->S transition. This effect was strictly limited to the pressed area of the skin. The proto-oncogene c-fos was induced within the first 2 min following application of the pressure. The level of c-fos mRNA showed two peaks during the next 24 h. The first slight peak was preceded by a rapid increase in the cAMP level in the pressed skin, the second-by a fall in the cAMP concentration. A model is suggested to explain the observed effects by reversible functional damage of the cellular membrane affecting the enzymes maintaining the steady state level of cAMP.
ISSN:0001-527X
1734-154X
DOI:10.18388/abp.1996_4455