A novel TTX-producing Aeromonas isolated from the ovary of Takifugu obscurus
Puffer fish ( Takifugu obscurus) from the Yangtze River of China were screened for tetrodotoxin-producing bacteria. An Aeromonas strain was isolated from the ovary of the puffer fish and was shown to produce tetrodotoxin; this strain was denoted Ne-1. The identity of tetrodotoxin produced by strain...
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Veröffentlicht in: | Toxicon (Oxford) 2010-09, Vol.56 (3), p.324-329 |
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Sprache: | eng |
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Zusammenfassung: | Puffer fish (
Takifugu obscurus) from the Yangtze River of China were screened for tetrodotoxin-producing bacteria. An
Aeromonas strain was isolated from the ovary of the puffer fish and was shown to produce tetrodotoxin; this strain was denoted Ne-1. The identity of tetrodotoxin produced by strain Ne-1 was confirmed by mouse bioassay, high performance liquid chromatography coupled with mass chromatography (LC-MS), and ELISA. Strain Ne-1 was characterized morphologically, biochemically, and by 16S rDNA phylogenetic analysis; these analyses suggested that strain Ne-1 is closely related to
Aeromonas molluscorum. Given that strain Ne-1 was isolated from the ovary of
T.
obscurus, we propose that the TTX-producing
Aeromonas sp. is a parasite or symbiotic bacterium rather than a sample contaminant. Collectively, our studies suggest that
Aeromonas sp. strain Ne-1 produces tetrodotoxin in
T.
obscurus. |
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ISSN: | 0041-0101 1879-3150 |
DOI: | 10.1016/j.toxicon.2010.03.019 |