A Simple Improved Method for the Measurement of Cyclosporin by Liquid-Liquid Extraction of Whole Blood and Isocratic HPLC

The current HPLC methods of cyclosporin measurement have been reviewed and all aspects assessed. A simple isocratic C-18 reverse phase HPLC method with improved efficiency is described for the routine measurement of cyclosporin in whole blood. An alkaline ether extraction is followed by an acid wash...

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Veröffentlicht in:Annals of clinical biochemistry 1988-09, Vol.25 (5), p.510-515
Hauptverfasser: Fletcher, Stephen J, Bacchus, Robby A
Format: Artikel
Sprache:eng
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Zusammenfassung:The current HPLC methods of cyclosporin measurement have been reviewed and all aspects assessed. A simple isocratic C-18 reverse phase HPLC method with improved efficiency is described for the routine measurement of cyclosporin in whole blood. An alkaline ether extraction is followed by an acid wash, solvent evaporation and two hexane washes of the reconstituted extract. The turn-round time for a single sample is 1 h. Daily batches of up to 40 patient samples can be easily measured with this method. The results are compared with those from the Sandoz radioimmunoassay (RIA) method.
ISSN:0004-5632
1758-1001
DOI:10.1177/000456328802500506