Characterization of enzootic nasal tumor virus capsid antigen

The RT-PCR was carried out on tumor tissue from sheep with enzootic nasal tumor (ENT), using primers designed from conserved amino acid regions of related type D retroviruses. A 591 bp PCR fragment, corresponding to 90% of the capsid antigen was cloned, sequenced and expressed in E. coli. Alignment...

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Veröffentlicht in:	Veterinary microbiology 1996-12, Vol.53 (3), p.261-269
Hauptverfasser:	Rosati, S., Kwang, J., Tolari, F., Keen, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Animal viral diseases Animals Antibodies, Monoclonal ANTIGENE ANTIGENOS ANTIGENS Antigens, Viral - analysis Antigens, Viral - genetics Base Sequence Betaretrovirus - genetics Betaretrovirus - isolation & purification Biological and medical sciences Capsid - analysis Capsid - genetics Capsid antigen Cloning, Molecular Enzootic nasal tumor Enzyme-Linked Immunosorbent Assay Escherichia coli Infectious diseases Lung Neoplasms - veterinary Lung Neoplasms - virology Mason-Pfizer monkey virus - genetics Medical sciences Mice Molecular Sequence Data NEOPLASMAS NEOPLASME NEOPLASMS Nose Neoplasms - diagnosis Nose Neoplasms - veterinary Nose Neoplasms - virology OVIN OVINOS PCR Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Recombinant RETROVIRIDAE Retroviridae Infections - diagnosis Retroviridae Infections - veterinary Retroviridae Infections - virology Sequence Alignment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid SHEEP Sheep Diseases Tumor Virus Infections - diagnosis Tumor Virus Infections - veterinary Tumor Virus Infections - virology Type-D retrovirus Viral diseases
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Beschreibung
Zusammenfassung:	The RT-PCR was carried out on tumor tissue from sheep with enzootic nasal tumor (ENT), using primers designed from conserved amino acid regions of related type D retroviruses. A 591 bp PCR fragment, corresponding to 90% of the capsid antigen was cloned, sequenced and expressed in E. coli. Alignment with ovine pulmonary carcinoma (OPC) virus showed 93% nucleotide and 96% amino acid homology. No amplification occurred when DNA from ovine fetal cell line was used as template. The recombinant protein, highly expressed in a prokaryotic system, reacted in immunoblot with mouse antiserum to Mason Pfizer monkey virus (MPMV) p27, as well as sera from OPC and ENT diseased animals. Preliminary application of this antigen in ELISA suggested its potential use to detect seropositive animals in infected flocks.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/S0378-1135(96)01218-7