Determination of Fat-Soluble Vitamins in a Pharmaceutical Dosage Form by Solid-Phase Extraction and Reversed-Phase Liquid Chromatography
A rapid and precise method for the determination of fat-soluble vitamins from a water-based multivitamin mixture was developed utilizing solid-phase extraction and reversed-phase high-performance liquid Chromatography (HPLC). Cholecalciferol. α-tocopherol acetate, and retinol palmitate were extracte...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 1988-09, Vol.77 (9), p.802-803 |
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| container_title | Journal of pharmaceutical sciences |
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| creator | Savolainen, K.E. Pynnönen, K.M. Lapinjoki, S.P. Vidgren, M.T. |
| description | A rapid and precise method for the determination of fat-soluble vitamins from a water-based multivitamin mixture was developed utilizing solid-phase extraction and reversed-phase high-performance liquid Chromatography (HPLC). Cholecalciferol. α-tocopherol acetate, and retinol palmitate were extracted in a single stage from the matrix using Bond Elut C18 columns The vitamins were then chromatographed on a Hypersil 5-μm C18 column, using a water; methanol gradient, and quantified simultaneously using individual UV absorption maxima. The recovery of added analytes varied from 92.6 to 100.6%. The assay coefficient of variation was 1.7–2.7%. The sample preparation method and HPLC assay described here are practical for pharmaceutical quality control purposes. |
| doi_str_mv | 10.1002/jps.2600770918 |
| format | Article |
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Cholecalciferol. α-tocopherol acetate, and retinol palmitate were extracted in a single stage from the matrix using Bond Elut C18 columns The vitamins were then chromatographed on a Hypersil 5-μm C18 column, using a water; methanol gradient, and quantified simultaneously using individual UV absorption maxima. The recovery of added analytes varied from 92.6 to 100.6%. The assay coefficient of variation was 1.7–2.7%. The sample preparation method and HPLC assay described here are practical for pharmaceutical quality control purposes.</description><identifier>ISSN: 0022-3549</identifier><identifier>EISSN: 1520-6017</identifier><identifier>DOI: 10.1002/jps.2600770918</identifier><identifier>PMID: 2852248</identifier><identifier>CODEN: JPMSAE</identifier><language>eng</language><publisher>Washington: Elsevier Inc</publisher><subject>Analysis ; Biological and medical sciences ; Cholecalciferol - analysis ; Chromatography, High Pressure Liquid ; Dosage Forms ; General pharmacology ; Medical sciences ; Pharmacology. Drug treatments ; Spectrophotometry, Ultraviolet ; Vitamin A - analogs & derivatives ; Vitamin A - analysis ; Vitamin E - analysis ; Vitamins - administration & dosage ; Vitamins - analysis</subject><ispartof>Journal of pharmaceutical sciences, 1988-09, Vol.77 (9), p.802-803</ispartof><rights>1988 Wiley-Liss, Inc., A Wiley Company</rights><rights>Copyright © 1988 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5228-dd8c369248cbb6f700594780bbb45816fd7ec8863859dac7da09753668878c3b3</citedby><cites>FETCH-LOGICAL-c5228-dd8c369248cbb6f700594780bbb45816fd7ec8863859dac7da09753668878c3b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjps.2600770918$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjps.2600770918$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7011097$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2852248$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Savolainen, K.E.</creatorcontrib><creatorcontrib>Pynnönen, K.M.</creatorcontrib><creatorcontrib>Lapinjoki, S.P.</creatorcontrib><creatorcontrib>Vidgren, M.T.</creatorcontrib><title>Determination of Fat-Soluble Vitamins in a Pharmaceutical Dosage Form by Solid-Phase Extraction and Reversed-Phase Liquid Chromatography</title><title>Journal of pharmaceutical sciences</title><addtitle>J. Pharm. Sci</addtitle><description>A rapid and precise method for the determination of fat-soluble vitamins from a water-based multivitamin mixture was developed utilizing solid-phase extraction and reversed-phase high-performance liquid Chromatography (HPLC). Cholecalciferol. α-tocopherol acetate, and retinol palmitate were extracted in a single stage from the matrix using Bond Elut C18 columns The vitamins were then chromatographed on a Hypersil 5-μm C18 column, using a water; methanol gradient, and quantified simultaneously using individual UV absorption maxima. The recovery of added analytes varied from 92.6 to 100.6%. The assay coefficient of variation was 1.7–2.7%. The sample preparation method and HPLC assay described here are practical for pharmaceutical quality control purposes.</description><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Cholecalciferol - analysis</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dosage Forms</subject><subject>General pharmacology</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Vitamin A - analogs & derivatives</subject><subject>Vitamin A - analysis</subject><subject>Vitamin E - analysis</subject><subject>Vitamins - administration & dosage</subject><subject>Vitamins - analysis</subject><issn>0022-3549</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1vEzEYhFcIVELhyg3JB9TbBns3_tgjSpq2UQQV5eNoee13G5fddWp7S_MP-rNx2RDEAXHyYZ6ZdzxZ9prgKcG4eHezDdOCYcw5roh4kk0ILXDOMOFPs0kCiryks-p59iKEG4wxw5QeZUeFoEUxE5PsYQERfGd7Fa3rkWvQUsX8yrVD3QL6aqNKWkC2RwpdbpTvlIYhWq1atHBBXQNaOt-heoeSx5o8MQHQ6X30Sv9KVL1Bn-AOfIDf6treDtag-ca7TkV37dV2s3uZPWtUG-DV_j3OvixPP8_P8_XHs4v5-3WuU2ORGyN0yarUXdc1azjGtJpxgeu6nlFBWGM4aCFYKWhllOZG4YrTkjEheHLW5XF2MuZuvbsdIETZ2aChbVUPbgiSC8YLXLEETkdQexeCh0Zuve2U30mC5eP0Mk0v_0yfDG_2yUPdgTng-62T_navq5D2a7zqtQ0HjGNCUteEVSP2w7aw-89Rubq8-qtCPnptiHB_8Cr_XTJeciq_fTiTK7oi54v1XD7eEiMPafE7C14GbaHXYKwHHaVx9l-__QkZdL9k</recordid><startdate>198809</startdate><enddate>198809</enddate><creator>Savolainen, K.E.</creator><creator>Pynnönen, K.M.</creator><creator>Lapinjoki, S.P.</creator><creator>Vidgren, M.T.</creator><general>Elsevier Inc</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>American Pharmaceutical Association</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198809</creationdate><title>Determination of Fat-Soluble Vitamins in a Pharmaceutical Dosage Form by Solid-Phase Extraction and Reversed-Phase Liquid Chromatography</title><author>Savolainen, K.E. ; Pynnönen, K.M. ; Lapinjoki, S.P. ; Vidgren, M.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5228-dd8c369248cbb6f700594780bbb45816fd7ec8863859dac7da09753668878c3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Cholecalciferol - analysis</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dosage Forms</topic><topic>General pharmacology</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Vitamin A - analogs & derivatives</topic><topic>Vitamin A - analysis</topic><topic>Vitamin E - analysis</topic><topic>Vitamins - administration & dosage</topic><topic>Vitamins - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Savolainen, K.E.</creatorcontrib><creatorcontrib>Pynnönen, K.M.</creatorcontrib><creatorcontrib>Lapinjoki, S.P.</creatorcontrib><creatorcontrib>Vidgren, M.T.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Savolainen, K.E.</au><au>Pynnönen, K.M.</au><au>Lapinjoki, S.P.</au><au>Vidgren, M.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Fat-Soluble Vitamins in a Pharmaceutical Dosage Form by Solid-Phase Extraction and Reversed-Phase Liquid Chromatography</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><addtitle>J. Pharm. Sci</addtitle><date>1988-09</date><risdate>1988</risdate><volume>77</volume><issue>9</issue><spage>802</spage><epage>803</epage><pages>802-803</pages><issn>0022-3549</issn><eissn>1520-6017</eissn><coden>JPMSAE</coden><abstract>A rapid and precise method for the determination of fat-soluble vitamins from a water-based multivitamin mixture was developed utilizing solid-phase extraction and reversed-phase high-performance liquid Chromatography (HPLC). Cholecalciferol. α-tocopherol acetate, and retinol palmitate were extracted in a single stage from the matrix using Bond Elut C18 columns The vitamins were then chromatographed on a Hypersil 5-μm C18 column, using a water; methanol gradient, and quantified simultaneously using individual UV absorption maxima. The recovery of added analytes varied from 92.6 to 100.6%. The assay coefficient of variation was 1.7–2.7%. The sample preparation method and HPLC assay described here are practical for pharmaceutical quality control purposes.</abstract><cop>Washington</cop><pub>Elsevier Inc</pub><pmid>2852248</pmid><doi>10.1002/jps.2600770918</doi><tpages>2</tpages></addata></record> |
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| ispartof | Journal of pharmaceutical sciences, 1988-09, Vol.77 (9), p.802-803 |
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| language | eng |
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| source | MEDLINE; Wiley Journals; Alma/SFX Local Collection |
| subjects | Analysis Biological and medical sciences Cholecalciferol - analysis Chromatography, High Pressure Liquid Dosage Forms General pharmacology Medical sciences Pharmacology. Drug treatments Spectrophotometry, Ultraviolet Vitamin A - analogs & derivatives Vitamin A - analysis Vitamin E - analysis Vitamins - administration & dosage Vitamins - analysis |
| title | Determination of Fat-Soluble Vitamins in a Pharmaceutical Dosage Form by Solid-Phase Extraction and Reversed-Phase Liquid Chromatography |
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