Determination of Fat-Soluble Vitamins in a Pharmaceutical Dosage Form by Solid-Phase Extraction and Reversed-Phase Liquid Chromatography
A rapid and precise method for the determination of fat-soluble vitamins from a water-based multivitamin mixture was developed utilizing solid-phase extraction and reversed-phase high-performance liquid Chromatography (HPLC). Cholecalciferol. α-tocopherol acetate, and retinol palmitate were extracte...
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Veröffentlicht in: | Journal of pharmaceutical sciences 1988-09, Vol.77 (9), p.802-803 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A rapid and precise method for the determination of fat-soluble vitamins from a water-based multivitamin mixture was developed utilizing solid-phase extraction and reversed-phase high-performance liquid Chromatography (HPLC). Cholecalciferol. α-tocopherol acetate, and retinol palmitate were extracted in a single stage from the matrix using Bond Elut C18 columns The vitamins were then chromatographed on a Hypersil 5-μm C18 column, using a water; methanol gradient, and quantified simultaneously using individual UV absorption maxima. The recovery of added analytes varied from 92.6 to 100.6%. The assay coefficient of variation was 1.7–2.7%. The sample preparation method and HPLC assay described here are practical for pharmaceutical quality control purposes. |
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ISSN: | 0022-3549 1520-6017 |
DOI: | 10.1002/jps.2600770918 |