Characterisation of genes involved in biosynthesis of coronafacic acid, the polyketide component of the phytotoxin coronatine
Coronafacic acid (CFA) is the polyketide component of coronatine (COR), a phytotoxin produced by the plant pathogen, Pseudomonas syringae. In the present study we have determined the nucleotide sequence of a 3.92-kb DNA fragment involved in CFA biosynthesis. Analysis of the sequence revealed four co...
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| Veröffentlicht in: | Gene 1996, Vol.183 (1), p.167-173 |
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| creator | Penfold, C.N. Bender, C.L. Turner, J.G. |
| description | Coronafacic acid (CFA) is the polyketide component of coronatine (COR), a phytotoxin produced by the plant pathogen,
Pseudomonas syringae. In the present study we have determined the nucleotide sequence of a 3.92-kb DNA fragment involved in CFA biosynthesis. Analysis of the sequence revealed four complete open reading frames (ORFs) designated
cfal to
cfa4 and one incomplete ORF (
cfa5), all transcribed in the same direction. The predicted translation products of
cfal, cfa2 and
cfa3 showed relatedness to acyl carrier proteins, fatty acid dehydrases and β-ketoacylsynthases, respectively, which are required for polyketide synthesis.
cfal was subcloned, its sequence was confirmed, and it was overexpressed in
E. coli to yield a peptide with an apparent molecular mass of 6 kDa. |
| doi_str_mv | 10.1016/S0378-1119(96)00550-1 |
| format | Article |
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Pseudomonas syringae. In the present study we have determined the nucleotide sequence of a 3.92-kb DNA fragment involved in CFA biosynthesis. Analysis of the sequence revealed four complete open reading frames (ORFs) designated
cfal to
cfa4 and one incomplete ORF (
cfa5), all transcribed in the same direction. The predicted translation products of
cfal, cfa2 and
cfa3 showed relatedness to acyl carrier proteins, fatty acid dehydrases and β-ketoacylsynthases, respectively, which are required for polyketide synthesis.
cfal was subcloned, its sequence was confirmed, and it was overexpressed in
E. coli to yield a peptide with an apparent molecular mass of 6 kDa.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(96)00550-1</identifier><identifier>PMID: 8996103</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acyl carrier protein, acyltransferase ; acyl carrier proteins ; acyltransferases ; Amino Acid Sequence ; amino acid sequences ; Amino Acids - metabolism ; bacterial proteins ; bacterial toxins ; Bacterial Toxins - metabolism ; Base Sequence ; beta-ketoacylsynthase ; biosynthesis ; cfa1 gene ; cfa2 gene ; cfa3 gene ; cfa4 gene ; cfa5 gene ; Cloning, Molecular ; Coronafacic acid ; Escherichia coli - genetics ; fatty acid dehydrase ; genbank/u56980 ; Gene Expression ; Genes, Bacterial - genetics ; Indenes - metabolism ; Molecular Sequence Data ; Molecular Weight ; Multienzyme Complexes - genetics ; nucleotide sequences ; open reading frames ; Open Reading Frames - genetics ; polyketide synthase ; Pseudomonas - enzymology ; Pseudomonas - genetics ; Pseudomonas syringae ; Pseudomonas syringae pv. glycinea ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - chemistry ; Restriction Mapping ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; structural genes ; Type II polyketide synthase ; β-Ketoacylsynthase, dehydrase</subject><ispartof>Gene, 1996, Vol.183 (1), p.167-173</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-67bd6b3f1a587763df61bef02818efac5a02c32f15d627339d5e2c272064c0b23</citedby><cites>FETCH-LOGICAL-c415t-67bd6b3f1a587763df61bef02818efac5a02c32f15d627339d5e2c272064c0b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1119(96)00550-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,4025,27928,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8996103$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Penfold, C.N.</creatorcontrib><creatorcontrib>Bender, C.L.</creatorcontrib><creatorcontrib>Turner, J.G.</creatorcontrib><title>Characterisation of genes involved in biosynthesis of coronafacic acid, the polyketide component of the phytotoxin coronatine</title><title>Gene</title><addtitle>Gene</addtitle><description>Coronafacic acid (CFA) is the polyketide component of coronatine (COR), a phytotoxin produced by the plant pathogen,
Pseudomonas syringae. In the present study we have determined the nucleotide sequence of a 3.92-kb DNA fragment involved in CFA biosynthesis. Analysis of the sequence revealed four complete open reading frames (ORFs) designated
cfal to
cfa4 and one incomplete ORF (
cfa5), all transcribed in the same direction. The predicted translation products of
cfal, cfa2 and
cfa3 showed relatedness to acyl carrier proteins, fatty acid dehydrases and β-ketoacylsynthases, respectively, which are required for polyketide synthesis.
cfal was subcloned, its sequence was confirmed, and it was overexpressed in
E. coli to yield a peptide with an apparent molecular mass of 6 kDa.</description><subject>Acyl carrier protein, acyltransferase</subject><subject>acyl carrier proteins</subject><subject>acyltransferases</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Amino Acids - metabolism</subject><subject>bacterial proteins</subject><subject>bacterial toxins</subject><subject>Bacterial Toxins - metabolism</subject><subject>Base Sequence</subject><subject>beta-ketoacylsynthase</subject><subject>biosynthesis</subject><subject>cfa1 gene</subject><subject>cfa2 gene</subject><subject>cfa3 gene</subject><subject>cfa4 gene</subject><subject>cfa5 gene</subject><subject>Cloning, Molecular</subject><subject>Coronafacic acid</subject><subject>Escherichia coli - genetics</subject><subject>fatty acid dehydrase</subject><subject>genbank/u56980</subject><subject>Gene Expression</subject><subject>Genes, Bacterial - genetics</subject><subject>Indenes - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Multienzyme Complexes - genetics</subject><subject>nucleotide sequences</subject><subject>open reading frames</subject><subject>Open Reading Frames - genetics</subject><subject>polyketide synthase</subject><subject>Pseudomonas - enzymology</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas syringae</subject><subject>Pseudomonas syringae pv. glycinea</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Restriction Mapping</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>structural genes</subject><subject>Type II polyketide synthase</subject><subject>β-Ketoacylsynthase, dehydrase</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vEzEQxVcIVNLCR6jYE6ISCx67ttenCkX8kypxKD1bXnu2MSR2sJ2IHPjueLNRr72MR3q_Nx7Na5pLIB-AgPh4R5jsOwBQ75S4IoRz0sGzZgG9VB0hrH_eLB6Rl815zr8ImTB61pz1SgkgbNH8W65MMrZg8tkUH0Mbx_YBA-bWh31c79HVph18zIdQVph9nggbUwxmNNbbthb3vq1au43rw28s3mEFNtsYMJSJPmqrQ4kl_q3DZnPxAV81L0azzvj69F40918-_1x-625_fP2-_HTb2WvgpRNycGJgIxjeSymYGwUMOBLaQ491CW4ItYyOwJ2gkjHlOFJLJSXi2pKBsovm7Tx3m-KfHeaiNz5bXK9NwLjLWvZCUCLlkyBwqRgHVkE-gzbFnBOOepv8xqSDBqKnfPQxHz0dXyuhj_loqL7L0we7YYPu0XUKpOpvZn00UZuHmoq-v6MEGAFOuVKqEjczgfVge49JZ-sxWHQ-oS3aRf_EDv8BZn2rDQ</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Penfold, C.N.</creator><creator>Bender, C.L.</creator><creator>Turner, J.G.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1996</creationdate><title>Characterisation of genes involved in biosynthesis of coronafacic acid, the polyketide component of the phytotoxin coronatine</title><author>Penfold, C.N. ; Bender, C.L. ; Turner, J.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-67bd6b3f1a587763df61bef02818efac5a02c32f15d627339d5e2c272064c0b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Acyl carrier protein, acyltransferase</topic><topic>acyl carrier proteins</topic><topic>acyltransferases</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Amino Acids - metabolism</topic><topic>bacterial proteins</topic><topic>bacterial toxins</topic><topic>Bacterial Toxins - metabolism</topic><topic>Base Sequence</topic><topic>beta-ketoacylsynthase</topic><topic>biosynthesis</topic><topic>cfa1 gene</topic><topic>cfa2 gene</topic><topic>cfa3 gene</topic><topic>cfa4 gene</topic><topic>cfa5 gene</topic><topic>Cloning, Molecular</topic><topic>Coronafacic acid</topic><topic>Escherichia coli - genetics</topic><topic>fatty acid dehydrase</topic><topic>genbank/u56980</topic><topic>Gene Expression</topic><topic>Genes, Bacterial - genetics</topic><topic>Indenes - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Multienzyme Complexes - genetics</topic><topic>nucleotide sequences</topic><topic>open reading frames</topic><topic>Open Reading Frames - genetics</topic><topic>polyketide synthase</topic><topic>Pseudomonas - enzymology</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas syringae</topic><topic>Pseudomonas syringae pv. glycinea</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - chemistry</topic><topic>Restriction Mapping</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>structural genes</topic><topic>Type II polyketide synthase</topic><topic>β-Ketoacylsynthase, dehydrase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Penfold, C.N.</creatorcontrib><creatorcontrib>Bender, C.L.</creatorcontrib><creatorcontrib>Turner, J.G.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Penfold, C.N.</au><au>Bender, C.L.</au><au>Turner, J.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation of genes involved in biosynthesis of coronafacic acid, the polyketide component of the phytotoxin coronatine</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1996</date><risdate>1996</risdate><volume>183</volume><issue>1</issue><spage>167</spage><epage>173</epage><pages>167-173</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Coronafacic acid (CFA) is the polyketide component of coronatine (COR), a phytotoxin produced by the plant pathogen,
Pseudomonas syringae. In the present study we have determined the nucleotide sequence of a 3.92-kb DNA fragment involved in CFA biosynthesis. Analysis of the sequence revealed four complete open reading frames (ORFs) designated
cfal to
cfa4 and one incomplete ORF (
cfa5), all transcribed in the same direction. The predicted translation products of
cfal, cfa2 and
cfa3 showed relatedness to acyl carrier proteins, fatty acid dehydrases and β-ketoacylsynthases, respectively, which are required for polyketide synthesis.
cfal was subcloned, its sequence was confirmed, and it was overexpressed in
E. coli to yield a peptide with an apparent molecular mass of 6 kDa.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8996103</pmid><doi>10.1016/S0378-1119(96)00550-1</doi><tpages>7</tpages></addata></record> |
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| subjects | Acyl carrier protein, acyltransferase acyl carrier proteins acyltransferases Amino Acid Sequence amino acid sequences Amino Acids - metabolism bacterial proteins bacterial toxins Bacterial Toxins - metabolism Base Sequence beta-ketoacylsynthase biosynthesis cfa1 gene cfa2 gene cfa3 gene cfa4 gene cfa5 gene Cloning, Molecular Coronafacic acid Escherichia coli - genetics fatty acid dehydrase genbank/u56980 Gene Expression Genes, Bacterial - genetics Indenes - metabolism Molecular Sequence Data Molecular Weight Multienzyme Complexes - genetics nucleotide sequences open reading frames Open Reading Frames - genetics polyketide synthase Pseudomonas - enzymology Pseudomonas - genetics Pseudomonas syringae Pseudomonas syringae pv. glycinea Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid structural genes Type II polyketide synthase β-Ketoacylsynthase, dehydrase |
| title | Characterisation of genes involved in biosynthesis of coronafacic acid, the polyketide component of the phytotoxin coronatine |
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