Characterisation of genes involved in biosynthesis of coronafacic acid, the polyketide component of the phytotoxin coronatine

Coronafacic acid (CFA) is the polyketide component of coronatine (COR), a phytotoxin produced by the plant pathogen, Pseudomonas syringae. In the present study we have determined the nucleotide sequence of a 3.92-kb DNA fragment involved in CFA biosynthesis. Analysis of the sequence revealed four co...

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Veröffentlicht in:	Gene 1996, Vol.183 (1), p.167-173
Hauptverfasser:	Penfold, C.N., Bender, C.L., Turner, J.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acyl carrier protein, acyltransferase acyl carrier proteins acyltransferases Amino Acid Sequence amino acid sequences Amino Acids - metabolism bacterial proteins bacterial toxins Bacterial Toxins - metabolism Base Sequence beta-ketoacylsynthase biosynthesis cfa1 gene cfa2 gene cfa3 gene cfa4 gene cfa5 gene Cloning, Molecular Coronafacic acid Escherichia coli - genetics fatty acid dehydrase genbank/u56980 Gene Expression Genes, Bacterial - genetics Indenes - metabolism Molecular Sequence Data Molecular Weight Multienzyme Complexes - genetics nucleotide sequences open reading frames Open Reading Frames - genetics polyketide synthase Pseudomonas - enzymology Pseudomonas - genetics Pseudomonas syringae Pseudomonas syringae pv. glycinea Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid structural genes Type II polyketide synthase β-Ketoacylsynthase, dehydrase
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Zusammenfassung:	Coronafacic acid (CFA) is the polyketide component of coronatine (COR), a phytotoxin produced by the plant pathogen, Pseudomonas syringae. In the present study we have determined the nucleotide sequence of a 3.92-kb DNA fragment involved in CFA biosynthesis. Analysis of the sequence revealed four complete open reading frames (ORFs) designated cfal to cfa4 and one incomplete ORF ( cfa5), all transcribed in the same direction. The predicted translation products of cfal, cfa2 and cfa3 showed relatedness to acyl carrier proteins, fatty acid dehydrases and β-ketoacylsynthases, respectively, which are required for polyketide synthesis. cfal was subcloned, its sequence was confirmed, and it was overexpressed in E. coli to yield a peptide with an apparent molecular mass of 6 kDa.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/S0378-1119(96)00550-1