Quantitation of infectious human immunodeficiency virus using a modified plaque forming assay
This report describes a modified plaque forming assay which has been used for quantitation of infectious human immunodeficiency virus (HIV) in cell culture supernatant fluid. The number of infectious units determined by the plaque assay correlates closely with the 50% infectious dose determined by a...
Gespeichert in:
| Veröffentlicht in: | Journal of virological methods 1988-12, Vol.22 (2), p.303-308 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 308 |
|---|---|
| container_issue | 2 |
| container_start_page | 303 |
| container_title | Journal of virological methods |
| container_volume | 22 |
| creator | Kiernan, R.E. McPhee, D.A. Doherty, R.R. |
| description | This report describes a modified plaque forming assay which has been used for quantitation of infectious human immunodeficiency virus (HIV) in cell culture supernatant fluid. The number of infectious units determined by the plaque assay correlates closely with the 50% infectious dose determined by a conventional assay in cell culture. In contrast, particle associated reverse transcriptase (RT) activity correlates poorly with the amount of infectious HIV present in culture supernatant fluids. |
| doi_str_mv | 10.1016/0166-0934(88)90112-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78658891</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0166093488901127</els_id><sourcerecordid>15123383</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-ad2274ba96b807012315e33a107f6bdc69d92c50c994e7dbc51d3bc9a59435fd3</originalsourceid><addsrcrecordid>eNqFkVtLHTEUhUOx2KPtP1DIgxT7MDaZ3F8EEa0FQQT7WEImF02ZyZwmMwfOv2_OhfNYH0JI1tpr73wB4AyjK4ww_14Xb5Ai9FLKbwph3DbiA1hgKVS9lvQILA6WT-CklD8IISYIOQbHLeWUI7UAv59nk6Y4mSmOCY4BxhS8rYe5wLd5MAnGYZjT6HyINvpk13AVcxXnEtMrNHAYXQzRO7jszd_ZwzDmYauUYtafwcdg-uK_7PdT8Ov-7uX2oXl8-vHz9uaxsRSLqTGubQXtjOKdRALhlmDmCTEYicA7Z7lyqrUMWaWoF66zDDvSWWWYooQFR07B113uMo91iDLpIRbr-94kX1-iheRMSoXfNWJWmxNJqpHujDaPpWQf9DLHweS1xkhv8OsNW71hq6XUW_xa1LLzff7cDd4diva8q36x102xpg_ZJBvLwSZa1hK-ibne2XyFtoo-67KF713M9Xe0G-P_5_gHBCGh1w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15123383</pqid></control><display><type>article</type><title>Quantitation of infectious human immunodeficiency virus using a modified plaque forming assay</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Kiernan, R.E. ; McPhee, D.A. ; Doherty, R.R.</creator><creatorcontrib>Kiernan, R.E. ; McPhee, D.A. ; Doherty, R.R.</creatorcontrib><description>This report describes a modified plaque forming assay which has been used for quantitation of infectious human immunodeficiency virus (HIV) in cell culture supernatant fluid. The number of infectious units determined by the plaque assay correlates closely with the 50% infectious dose determined by a conventional assay in cell culture. In contrast, particle associated reverse transcriptase (RT) activity correlates poorly with the amount of infectious HIV present in culture supernatant fluids.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/0166-0934(88)90112-7</identifier><identifier>PMID: 2464609</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>AIDS/HIV ; Biological and medical sciences ; Cell Line ; Cytopathogenic Effect, Viral ; Fundamental and applied biological sciences. Psychology ; HIV ; HIV - enzymology ; HIV - growth & development ; HIV - isolation & purification ; human immunodeficiency virus ; Humans ; Microbiology ; Plaque assay ; Quantitation ; RNA-Directed DNA Polymerase - analysis ; Techniques used in virology ; Viral Plaque Assay ; Virology</subject><ispartof>Journal of virological methods, 1988-12, Vol.22 (2), p.303-308</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-ad2274ba96b807012315e33a107f6bdc69d92c50c994e7dbc51d3bc9a59435fd3</citedby><cites>FETCH-LOGICAL-c417t-ad2274ba96b807012315e33a107f6bdc69d92c50c994e7dbc51d3bc9a59435fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0166093488901127$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7252367$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2464609$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kiernan, R.E.</creatorcontrib><creatorcontrib>McPhee, D.A.</creatorcontrib><creatorcontrib>Doherty, R.R.</creatorcontrib><title>Quantitation of infectious human immunodeficiency virus using a modified plaque forming assay</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>This report describes a modified plaque forming assay which has been used for quantitation of infectious human immunodeficiency virus (HIV) in cell culture supernatant fluid. The number of infectious units determined by the plaque assay correlates closely with the 50% infectious dose determined by a conventional assay in cell culture. In contrast, particle associated reverse transcriptase (RT) activity correlates poorly with the amount of infectious HIV present in culture supernatant fluids.</description><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cytopathogenic Effect, Viral</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIV</subject><subject>HIV - enzymology</subject><subject>HIV - growth & development</subject><subject>HIV - isolation & purification</subject><subject>human immunodeficiency virus</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Plaque assay</subject><subject>Quantitation</subject><subject>RNA-Directed DNA Polymerase - analysis</subject><subject>Techniques used in virology</subject><subject>Viral Plaque Assay</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVtLHTEUhUOx2KPtP1DIgxT7MDaZ3F8EEa0FQQT7WEImF02ZyZwmMwfOv2_OhfNYH0JI1tpr73wB4AyjK4ww_14Xb5Ai9FLKbwph3DbiA1hgKVS9lvQILA6WT-CklD8IISYIOQbHLeWUI7UAv59nk6Y4mSmOCY4BxhS8rYe5wLd5MAnGYZjT6HyINvpk13AVcxXnEtMrNHAYXQzRO7jszd_ZwzDmYauUYtafwcdg-uK_7PdT8Ov-7uX2oXl8-vHz9uaxsRSLqTGubQXtjOKdRALhlmDmCTEYicA7Z7lyqrUMWaWoF66zDDvSWWWYooQFR07B113uMo91iDLpIRbr-94kX1-iheRMSoXfNWJWmxNJqpHujDaPpWQf9DLHweS1xkhv8OsNW71hq6XUW_xa1LLzff7cDd4diva8q36x102xpg_ZJBvLwSZa1hK-ibne2XyFtoo-67KF713M9Xe0G-P_5_gHBCGh1w</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Kiernan, R.E.</creator><creator>McPhee, D.A.</creator><creator>Doherty, R.R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19881201</creationdate><title>Quantitation of infectious human immunodeficiency virus using a modified plaque forming assay</title><author>Kiernan, R.E. ; McPhee, D.A. ; Doherty, R.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-ad2274ba96b807012315e33a107f6bdc69d92c50c994e7dbc51d3bc9a59435fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cytopathogenic Effect, Viral</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIV</topic><topic>HIV - enzymology</topic><topic>HIV - growth & development</topic><topic>HIV - isolation & purification</topic><topic>human immunodeficiency virus</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Plaque assay</topic><topic>Quantitation</topic><topic>RNA-Directed DNA Polymerase - analysis</topic><topic>Techniques used in virology</topic><topic>Viral Plaque Assay</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiernan, R.E.</creatorcontrib><creatorcontrib>McPhee, D.A.</creatorcontrib><creatorcontrib>Doherty, R.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kiernan, R.E.</au><au>McPhee, D.A.</au><au>Doherty, R.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitation of infectious human immunodeficiency virus using a modified plaque forming assay</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>22</volume><issue>2</issue><spage>303</spage><epage>308</epage><pages>303-308</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>This report describes a modified plaque forming assay which has been used for quantitation of infectious human immunodeficiency virus (HIV) in cell culture supernatant fluid. The number of infectious units determined by the plaque assay correlates closely with the 50% infectious dose determined by a conventional assay in cell culture. In contrast, particle associated reverse transcriptase (RT) activity correlates poorly with the amount of infectious HIV present in culture supernatant fluids.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2464609</pmid><doi>10.1016/0166-0934(88)90112-7</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 1988-12, Vol.22 (2), p.303-308 |
| issn | 0166-0934 1879-0984 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78658891 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | AIDS/HIV Biological and medical sciences Cell Line Cytopathogenic Effect, Viral Fundamental and applied biological sciences. Psychology HIV HIV - enzymology HIV - growth & development HIV - isolation & purification human immunodeficiency virus Humans Microbiology Plaque assay Quantitation RNA-Directed DNA Polymerase - analysis Techniques used in virology Viral Plaque Assay Virology |
| title | Quantitation of infectious human immunodeficiency virus using a modified plaque forming assay |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T16%3A55%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitation%20of%20infectious%20human%20immunodeficiency%20virus%20using%20a%20modified%20plaque%20forming%20assay&rft.jtitle=Journal%20of%20virological%20methods&rft.au=Kiernan,%20R.E.&rft.date=1988-12-01&rft.volume=22&rft.issue=2&rft.spage=303&rft.epage=308&rft.pages=303-308&rft.issn=0166-0934&rft.eissn=1879-0984&rft.coden=JVMEDH&rft_id=info:doi/10.1016/0166-0934(88)90112-7&rft_dat=%3Cproquest_cross%3E15123383%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15123383&rft_id=info:pmid/2464609&rft_els_id=0166093488901127&rfr_iscdi=true |