Quantitation of infectious human immunodeficiency virus using a modified plaque forming assay

This report describes a modified plaque forming assay which has been used for quantitation of infectious human immunodeficiency virus (HIV) in cell culture supernatant fluid. The number of infectious units determined by the plaque assay correlates closely with the 50% infectious dose determined by a...

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Veröffentlicht in:	Journal of virological methods 1988-12, Vol.22 (2), p.303-308
Hauptverfasser:	Kiernan, R.E., McPhee, D.A., Doherty, R.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Biological and medical sciences Cell Line Cytopathogenic Effect, Viral Fundamental and applied biological sciences. Psychology HIV HIV - enzymology HIV - growth & development HIV - isolation & purification human immunodeficiency virus Humans Microbiology Plaque assay Quantitation RNA-Directed DNA Polymerase - analysis Techniques used in virology Viral Plaque Assay Virology
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Beschreibung
Zusammenfassung:	This report describes a modified plaque forming assay which has been used for quantitation of infectious human immunodeficiency virus (HIV) in cell culture supernatant fluid. The number of infectious units determined by the plaque assay correlates closely with the 50% infectious dose determined by a conventional assay in cell culture. In contrast, particle associated reverse transcriptase (RT) activity correlates poorly with the amount of infectious HIV present in culture supernatant fluids.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/0166-0934(88)90112-7