Cloning and nucleotide sequencing of the protease gene of Vibrio vulnificus

Cloning and nucleotide sequencing of the protease gene of Vibrio vulnificus

The gene ( vvp) encoding a thermolabile protease of Vibrio vulnificus was cloned and sequenced. The transcription start point was also determined by primer extension. The product of this gene is very likely the secretory neutral metalloprotease that has been purified and characterized previously.

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Bibliographische Detailangaben
Veröffentlicht in:Gene 1996-12, Vol.183 (1), p.255-257
Hauptverfasser: Cheng, Jaw-Chiou, Shao, Chung-Ping, Hor, Lien-I.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Bacterial Proteins
Base Sequence
Cloning, Molecular
Conserved zinc-binding region
Genes, Bacterial - genetics
Metalloendopeptidases - genetics
Molecular Sequence Data
Restriction Mapping
Sequence Analysis, DNA
Thermolabile protease
Transcription start point
Transcription, Genetic - genetics
Vibrio - enzymology
Vibrio - genetics
Vibrio vulnificus
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Beschreibung
Zusammenfassung:The gene ( vvp) encoding a thermolabile protease of Vibrio vulnificus was cloned and sequenced. The transcription start point was also determined by primer extension. The product of this gene is very likely the secretory neutral metalloprotease that has been purified and characterized previously.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(96)00488-X