Kinetics of taxol production and nutrient use in suspension cultures of Taxus cuspidata in shake flasks and a Wilson-type bioreactor
Suspension cultures of Taxus cuspidata were grown in shake flasks and Wilson-type reactors where bubbled air provided both agitation and mixing. Taxol titers of 22 mg l −1 were achieved for both configurations in 20 days for a volumetric productivity of 1.1 mg l −1 day −1. This productivity is many-...
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Veröffentlicht in: | Enzyme and microbial technology 1996-09, Vol.19 (4), p.256-260 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Suspension cultures of
Taxus cuspidata were grown in shake flasks and Wilson-type reactors where bubbled air provided both agitation and mixing. Taxol titers of 22 mg l
−1 were achieved for both configurations in 20 days for a volumetric productivity of 1.1 mg l
−1 day
−1. This productivity is many-fold higher than reported for other
Taxus sp. suspension cultures. Taxol was released to the extracellular medium as it was produced with little intracellular retention (⩽10%). Taxol production occurred during the last seven days of the cultivation period and was not growth-associated. Although the same taxol titers could be obtained in both reactor types, nutrient uptake rates were faster in the Wilson-type reactor than in shake flasks. Formation of a growth ring in the Wilson-type reactor reduced measured cell mass yields. |
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ISSN: | 0141-0229 1879-0909 |
DOI: | 10.1016/0141-0229(95)00243-X |