A mouse chromosome-specific YAC probe collection for in situ hybridization

A mouse chromosome-specific YAC probe collection for in situ hybridization

To facilitate the identification of mouse metaphase chromosomes by fluorescence in situ hybridization (FISH), a complete collection of mouse chromosome-specific markers has been established. Yeast artificial chromosome libraries were screened by polymerase chain reaction using primers for known loci...

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Veröffentlicht in:Genomics (San Diego, Calif.) Calif.), 1996-12, Vol.38 (3), p.432-434
Hauptverfasser: MONGELARD, F, PORAS, I, USSON, Y, BATTEUX, B, ROBERT-NICOUD, M, AVNER, P, VOURC'H, C
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Chromosome Mapping
Chromosomes - genetics
Chromosomes, Artificial, Yeast - genetics
Cytogenetics
Fundamental and applied biological sciences. Psychology
Gene Library
Genetic Markers
Genetics of eukaryotes. Biological and molecular evolution
In Situ Hybridization, Fluorescence
Mice - genetics
Polymerase Chain Reaction
Vertebrata
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Beschreibung
Zusammenfassung:To facilitate the identification of mouse metaphase chromosomes by fluorescence in situ hybridization (FISH), a complete collection of mouse chromosome-specific markers has been established. Yeast artificial chromosome libraries were screened by polymerase chain reaction using primers for known loci. DNAs from positive clones were then tested by FISH. One probe per chromosome was selected on the basis of high specificity (nonchimerism) and strong fluorescence.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.0649