Conditional Requirement for Sequences Distinct from the Replication Origin during Episomal Establishment of the BPV1 Genome

Conditional Requirement for Sequences Distinct from the Replication Origin during Episomal Establishment of the BPV1 Genome

Removal from BPV1 DNA of a short segment (nt. 4786-5045) that contains several protein binding sites and is required for efficient replication in short term assays prevents its autonomous maintenance in cell lines established by selection in G418 medium after cotransfer ofneor. In contrast, transfor...

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Veröffentlicht in:Biochemical and biophysical research communications 1996-12, Vol.229 (2), p.445-448
Hauptverfasser: Pierrefite, Valérie, Binetruy, Bernard, Rassoulzadegan, Minoo, Cuzin, François
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bovine papillomavirus 1 - genetics
Cell Line
DNA Replication
DNA, Recombinant
DNA, Viral
Genome, Viral
Plasmids
Rats
Replication Origin
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Zusammenfassung:Removal from BPV1 DNA of a short segment (nt. 4786-5045) that contains several protein binding sites and is required for efficient replication in short term assays prevents its autonomous maintenance in cell lines established by selection in G418 medium after cotransfer ofneor. In contrast, transformed cell lines established from foci, which express the viral genes at higher levels, maintain extrachromosomal copies of the deleted DNA. Two modes of maintenance of the viral genome are thus distinguished by their requirement for sequences in this region.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.1823