Cloning and sequencing the HinfI restriction and modification genes
The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI- AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes f...
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| Veröffentlicht in: | Gene 1988-10, Vol.70 (2), p.387-392 |
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| container_title | Gene |
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| creator | Chandrasegaran, Srinivasan Lunnen, Keith D. Smith, Hamilton O. Wilson, Geoffrey G. |
| description | The
HinfI restriction and modification genes were cloned on a 3.9-kb
PstI fragment inserted into the
PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb
BclI-
AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa). |
| doi_str_mv | 10.1016/0378-1119(88)90210-7 |
| format | Article |
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HinfI restriction and modification genes were cloned on a 3.9-kb
PstI fragment inserted into the
PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb
BclI-
AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(88)90210-7</identifier><identifier>PMID: 3063606</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; Deoxyribonucleases, Type II Site-Specific - genetics ; DNA, Bacterial ; Escherichia coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genes. Genome ; Haemophilus influenzae ; Haemophilus influenzae - genetics ; methylase ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Plasmids ; plasmids pBR322 and pUC19 ; recombinant DNA ; restriction endonuclease ; Restriction Mapping</subject><ispartof>Gene, 1988-10, Vol.70 (2), p.387-392</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-75e350069412a3557dc30a77044f344578d0fa0ff08efec4753ccf5e53b7aad3</citedby><cites>FETCH-LOGICAL-c417t-75e350069412a3557dc30a77044f344578d0fa0ff08efec4753ccf5e53b7aad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(88)90210-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7341646$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3063606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chandrasegaran, Srinivasan</creatorcontrib><creatorcontrib>Lunnen, Keith D.</creatorcontrib><creatorcontrib>Smith, Hamilton O.</creatorcontrib><creatorcontrib>Wilson, Geoffrey G.</creatorcontrib><title>Cloning and sequencing the HinfI restriction and modification genes</title><title>Gene</title><addtitle>Gene</addtitle><description>The
HinfI restriction and modification genes were cloned on a 3.9-kb
PstI fragment inserted into the
PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb
BclI-
AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonucleases, Type II Site-Specific - genetics</subject><subject>DNA, Bacterial</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genes. Genome</subject><subject>Haemophilus influenzae</subject><subject>Haemophilus influenzae - genetics</subject><subject>methylase</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>plasmids pBR322 and pUC19</subject><subject>recombinant DNA</subject><subject>restriction endonuclease</subject><subject>Restriction Mapping</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotX78A4U9iOhhdbJJNtmLIEVtoeCl95BmJzWyHzXZCv57d9vSo-YyZOaZl-Eh5IrCAwWaPwKTKqWUFndK3ReQUUjlERlTJYsUgKljMj4gp-Qsxk_onxDZiIwY5CyHfEwmk6ptfLNKTFMmEb822Njh231gMvWNmyUBYxe87XzbbKG6Lb3z1mwbK2wwXpATZ6qIl_t6ThavL4vJNJ2_v80mz_PUciq7VApkAiAvOM0ME0KWloGREjh3jHMhVQnOgHOg0KHlUjBrnUDBltKYkp2T213sOrT9nbHTtY8Wq8o02G6ilirPlFDyX5AKmlFZQA_yHWhDG2NAp9fB1yb8aAp6cKwHgXoQqJXSW8d6yL_e52-WNZaHpb3Ufn6zn5toTeWC6Z3GAyYZpzkfsKcdhr2zb49BR-t7_Vj6gLbTZev_vuMXhamWnA</recordid><startdate>19881030</startdate><enddate>19881030</enddate><creator>Chandrasegaran, Srinivasan</creator><creator>Lunnen, Keith D.</creator><creator>Smith, Hamilton O.</creator><creator>Wilson, Geoffrey G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19881030</creationdate><title>Cloning and sequencing the HinfI restriction and modification genes</title><author>Chandrasegaran, Srinivasan ; Lunnen, Keith D. ; Smith, Hamilton O. ; Wilson, Geoffrey G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-75e350069412a3557dc30a77044f344578d0fa0ff08efec4753ccf5e53b7aad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Deoxyribonucleases, Type II Site-Specific - genetics</topic><topic>DNA, Bacterial</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genes. Genome</topic><topic>Haemophilus influenzae</topic><topic>Haemophilus influenzae - genetics</topic><topic>methylase</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Plasmids</topic><topic>plasmids pBR322 and pUC19</topic><topic>recombinant DNA</topic><topic>restriction endonuclease</topic><topic>Restriction Mapping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chandrasegaran, Srinivasan</creatorcontrib><creatorcontrib>Lunnen, Keith D.</creatorcontrib><creatorcontrib>Smith, Hamilton O.</creatorcontrib><creatorcontrib>Wilson, Geoffrey G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chandrasegaran, Srinivasan</au><au>Lunnen, Keith D.</au><au>Smith, Hamilton O.</au><au>Wilson, Geoffrey G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and sequencing the HinfI restriction and modification genes</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1988-10-30</date><risdate>1988</risdate><volume>70</volume><issue>2</issue><spage>387</spage><epage>392</epage><pages>387-392</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>The
HinfI restriction and modification genes were cloned on a 3.9-kb
PstI fragment inserted into the
PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb
BclI-
AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3063606</pmid><doi>10.1016/0378-1119(88)90210-7</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1988-10, Vol.70 (2), p.387-392 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Cloning, Molecular Deoxyribonucleases, Type II Site-Specific - genetics DNA, Bacterial Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genes. Genome Haemophilus influenzae Haemophilus influenzae - genetics methylase Molecular and cellular biology Molecular genetics Molecular Sequence Data Plasmids plasmids pBR322 and pUC19 recombinant DNA restriction endonuclease Restriction Mapping |
| title | Cloning and sequencing the HinfI restriction and modification genes |
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