Cloning and sequencing the HinfI restriction and modification genes

The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI- AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes f...

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Veröffentlicht in:	Gene 1988-10, Vol.70 (2), p.387-392
Hauptverfasser:	Chandrasegaran, Srinivasan, Lunnen, Keith D., Smith, Hamilton O., Wilson, Geoffrey G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences Cloning, Molecular Deoxyribonucleases, Type II Site-Specific - genetics DNA, Bacterial Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genes. Genome Haemophilus influenzae Haemophilus influenzae - genetics methylase Molecular and cellular biology Molecular genetics Molecular Sequence Data Plasmids plasmids pBR322 and pUC19 recombinant DNA restriction endonuclease Restriction Mapping
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Beschreibung
Zusammenfassung:	The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI- AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(88)90210-7