Validation of a ribosomal DNA-polymerase chain reaction species diagnostic assay for the common malaria mosquito (Diptera: Culicidae) sibling species complex

A polymerase chain reaction method for identifying individuals in the Anopheles quadrimaculatus Say sibling species complex was validated for wild mosquitoes from Louisiana and Mississippi. This method distinguished An. quadrimaculatus species A, B, C, and D by detecting species-specific differences...

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Veröffentlicht in:	Journal of medical entomology 1996-11, Vol.33 (6), p.952-954
Hauptverfasser:	Rutledge, C.R. (Louisiana State University, Baton Rouge, LA.), Cornel, A.J, Meek, C.L, Collins, F.H
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN Animals Anopheles - genetics ANOPHELES QUADRIMACULATUS Biological and medical sciences CHIMIOTAXONOMIE Culicidae DNA, Ribosomal - analysis ESPECE ESPECIES FRATRIE Freshwater Fundamental and applied biological sciences. Psychology HERMANOS IDENTIFICACION IDENTIFICATION LOUISIANE LUISIANA Medically important nuisances and vectors, pests of stored products and materials: population survey and control MISSISSIPPI PCR Polymerase Chain Reaction - methods QUIMIOTAXONOMIA RIBOSOMAS RIBOSOME Vectors. Intermediate hosts
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Beschreibung
Zusammenfassung:	A polymerase chain reaction method for identifying individuals in the Anopheles quadrimaculatus Say sibling species complex was validated for wild mosquitoes from Louisiana and Mississippi. This method distinguished An. quadrimaculatus species A, B, C, and D by detecting species-specific differences in the 2nd internal transcribed spacer of ribosomal DNA and was 100% specific and 95% sensitive
ISSN:	0022-2585 1938-2928
DOI:	10.1093/jmedent/33.6.952