Expression of α v and β 3 Integrin Chains on Murine Lymphocytes
The vitronectin receptor is a member of the integrin family of adhesion protein receptors and binds a broad spectrum of ligands, including fibronectin and fibrinogen in addition to vitronectin. We have generated four mAbs that recognize the murine α vβ 3 vitronectin receptor. Biochemical and express...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1996-12, Vol.93 (25), p.14698-14703 |
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| container_end_page | 14703 |
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| container_issue | 25 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 93 |
| creator | Gerber, David J. Pereira, Pablo Huang, Shu Ying Pelletier, Claire Tonegawa, Susumu |
| description | The vitronectin receptor is a member of the integrin family of adhesion protein receptors and binds a broad spectrum of ligands, including fibronectin and fibrinogen in addition to vitronectin. We have generated four mAbs that recognize the murine α vβ 3 vitronectin receptor. Biochemical and expression analyses showed that two of the mAbs are specific for the α v chain, and two are specific for the β 3 chain. The mAbs are effective blocking reagents and inhibited cell adhesion to vitronectin, fibrinogen, and fibronectin. Staining analysis revealed expression of α v and β 3 on certain populations of murine thymocytes, splenocytes, and bone marrow cells. The expression of α v and β 3 appeared to be modulated at specific stages of thymocyte development, suggesting a possible function for the α vβ 3 vitronectin receptor in T cell development. |
| doi_str_mv | 10.1073/pnas.93.25.14698 |
| format | Article |
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We have generated four mAbs that recognize the murine α vβ 3 vitronectin receptor. Biochemical and expression analyses showed that two of the mAbs are specific for the α v chain, and two are specific for the β 3 chain. The mAbs are effective blocking reagents and inhibited cell adhesion to vitronectin, fibrinogen, and fibronectin. Staining analysis revealed expression of α v and β 3 on certain populations of murine thymocytes, splenocytes, and bone marrow cells. The expression of α v and β 3 appeared to be modulated at specific stages of thymocyte development, suggesting a possible function for the α vβ 3 vitronectin receptor in T cell development.</description><identifier>ISSN: 0027-8424</identifier><identifier>DOI: 10.1073/pnas.93.25.14698</identifier><identifier>PMID: 8962117</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antibodies ; Antibodies, Monoclonal ; Cellular immunity ; Flow Cytometry ; Hybridomas ; Integrins ; Lymphocyte Subsets - immunology ; Lymphocytes ; Mice ; Mice, Inbred C57BL ; Precipitin Tests ; Receptors ; Receptors, Vitronectin - analysis ; Receptors, Vitronectin - immunology ; T lymphocytes ; T-Lymphocytes - immunology ; Thymocytes ; Vitronectin receptors</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1996-12, Vol.93 (25), p.14698-14703</ispartof><rights>Copyright 1996 National Academy of Sciences</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41149$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41149$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8962117$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gerber, David J.</creatorcontrib><creatorcontrib>Pereira, Pablo</creatorcontrib><creatorcontrib>Huang, Shu Ying</creatorcontrib><creatorcontrib>Pelletier, Claire</creatorcontrib><creatorcontrib>Tonegawa, Susumu</creatorcontrib><title>Expression of α v and β 3 Integrin Chains on Murine Lymphocytes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The vitronectin receptor is a member of the integrin family of adhesion protein receptors and binds a broad spectrum of ligands, including fibronectin and fibrinogen in addition to vitronectin. We have generated four mAbs that recognize the murine α vβ 3 vitronectin receptor. Biochemical and expression analyses showed that two of the mAbs are specific for the α v chain, and two are specific for the β 3 chain. The mAbs are effective blocking reagents and inhibited cell adhesion to vitronectin, fibrinogen, and fibronectin. Staining analysis revealed expression of α v and β 3 on certain populations of murine thymocytes, splenocytes, and bone marrow cells. The expression of α v and β 3 appeared to be modulated at specific stages of thymocyte development, suggesting a possible function for the α vβ 3 vitronectin receptor in T cell development.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>Cellular immunity</subject><subject>Flow Cytometry</subject><subject>Hybridomas</subject><subject>Integrins</subject><subject>Lymphocyte Subsets - immunology</subject><subject>Lymphocytes</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Precipitin Tests</subject><subject>Receptors</subject><subject>Receptors, Vitronectin - analysis</subject><subject>Receptors, Vitronectin - immunology</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes - immunology</subject><subject>Thymocytes</subject><subject>Vitronectin receptors</subject><issn>0027-8424</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1OwzAYRT2ASinsCAnJE1uCf-PPY1W1UKmIpXtkJw5N1TghThB5LHiQPhORWrEyXV2doztchO4oiSlR_KnxJsSax0zGVCQaLtCUEKYiEExcoesQ9oQQLYFM0AR0wihVUzRffjWtC6GsPa4LfPzGn9j4HB9_MMdr37n3tvR4sTOlD3h0XvuxO7wZqmZXZ0Pnwg26LMwhuNtzztB2tdwuXqLN2_N6Md9EewrQRUkCGePWmkJplRcEHFgwVnBQORTCGimdYkwJ4yhQwXMOJLPCFLnMrKZ8hh5Ps01bf_QudGlVhswdDsa7ug-pgoQRCuRfkUoQSksxig9nsbeVy9OmLSvTDun5m5Hfn_g-dHX7hwWlQvNfY-duDg</recordid><startdate>19961210</startdate><enddate>19961210</enddate><creator>Gerber, David J.</creator><creator>Pereira, Pablo</creator><creator>Huang, Shu Ying</creator><creator>Pelletier, Claire</creator><creator>Tonegawa, Susumu</creator><general>National Academy of Sciences of the United States of America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>7TM</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19961210</creationdate><title>Expression of α v and β 3 Integrin Chains on Murine Lymphocytes</title><author>Gerber, David J. ; Pereira, Pablo ; Huang, Shu Ying ; Pelletier, Claire ; Tonegawa, Susumu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j188t-668c23bbaf797df08e8b8ab4387d8f4ba55e72274ae18143d380cb4afd5cb913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Cellular immunity</topic><topic>Flow Cytometry</topic><topic>Hybridomas</topic><topic>Integrins</topic><topic>Lymphocyte Subsets - immunology</topic><topic>Lymphocytes</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Precipitin Tests</topic><topic>Receptors</topic><topic>Receptors, Vitronectin - analysis</topic><topic>Receptors, Vitronectin - immunology</topic><topic>T lymphocytes</topic><topic>T-Lymphocytes - immunology</topic><topic>Thymocytes</topic><topic>Vitronectin receptors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gerber, David J.</creatorcontrib><creatorcontrib>Pereira, Pablo</creatorcontrib><creatorcontrib>Huang, Shu Ying</creatorcontrib><creatorcontrib>Pelletier, Claire</creatorcontrib><creatorcontrib>Tonegawa, Susumu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gerber, David J.</au><au>Pereira, Pablo</au><au>Huang, Shu Ying</au><au>Pelletier, Claire</au><au>Tonegawa, Susumu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of α v and β 3 Integrin Chains on Murine Lymphocytes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1996-12-10</date><risdate>1996</risdate><volume>93</volume><issue>25</issue><spage>14698</spage><epage>14703</epage><pages>14698-14703</pages><issn>0027-8424</issn><abstract>The vitronectin receptor is a member of the integrin family of adhesion protein receptors and binds a broad spectrum of ligands, including fibronectin and fibrinogen in addition to vitronectin. 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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1996-12, Vol.93 (25), p.14698-14703 |
| issn | 0027-8424 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies Antibodies, Monoclonal Cellular immunity Flow Cytometry Hybridomas Integrins Lymphocyte Subsets - immunology Lymphocytes Mice Mice, Inbred C57BL Precipitin Tests Receptors Receptors, Vitronectin - analysis Receptors, Vitronectin - immunology T lymphocytes T-Lymphocytes - immunology Thymocytes Vitronectin receptors |
| title | Expression of α v and β 3 Integrin Chains on Murine Lymphocytes |
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