Expression of α v and β 3 Integrin Chains on Murine Lymphocytes

The vitronectin receptor is a member of the integrin family of adhesion protein receptors and binds a broad spectrum of ligands, including fibronectin and fibrinogen in addition to vitronectin. We have generated four mAbs that recognize the murine α vβ 3 vitronectin receptor. Biochemical and expression analyses showed that two of the mAbs are specific for the α v chain, and two are specific for the β 3 chain. The mAbs are effective blocking reagents and inhibited cell adhesion to vitronectin, fibrinogen, and fibronectin. Staining analysis revealed expression of α v and β 3 on certain populations of murine thymocytes, splenocytes, and bone marrow cells. The expression of α v and β 3 appeared to be modulated at specific stages of thymocyte development, suggesting a possible function for the α vβ 3 vitronectin receptor in T cell development.