Hemoglobinometry: evaluation of a new method with a stable primary standard

Using purified chlorohemin as a stable standard, a newly described hemoglobinometry method was evaluated. All blood samples as well as chlorohemin gave identical absorption spectra in an alkaline-detergent solution with a broad peak at 575 nm. Both the reagent mixture and the chlorohemin standard we...

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Veröffentlicht in:Pathology 1988, Vol.20 (2), p.152-155
Hauptverfasser: Wylie, B.R., Lovric, V.A.
Format: Artikel
Sprache:eng
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Zusammenfassung:Using purified chlorohemin as a stable standard, a newly described hemoglobinometry method was evaluated. All blood samples as well as chlorohemin gave identical absorption spectra in an alkaline-detergent solution with a broad peak at 575 nm. Both the reagent mixture and the chlorohemin standard were stable for at least 250 days when stored between 8°C and 32°C in brown glass bottles. The new method showed close correlation when compared to the cyanmethemoglobin technique. Compared to the cyanmethemoglobin method the advantages included a stable standard, shorter conversion time, reduced plasma background and improved hemoglobin ligands conversion. We conclude that the new method confers significant advantages over the cyanmethemoglobin method and in particular provides a stable primary standard with a long shelf-life.
ISSN:0031-3025
1465-3931
DOI:10.3109/00313028809066626