Synthesis of alanine and leucine by reductive amination of 2-oxoic acid with combination of hydrogenase and dehydrogenase

Synthesis of alanine and leucine by reductive amination of 2-oxoic acid with combination of hydrogenase and dehydrogenase

Alanine synthesis by reductive amination of pyruvate was performed by the combination of NADH regeneration system and alanine dehydrogenase (AlaDH). The conversion of pyruvate to alanine was 99% after 1 h. Leucine synthesis was also carried out by the combination of NADH regeneration system and leuc...

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Veröffentlicht in:Applied biochemistry and biotechnology 1996-03, Vol.56 (3), p.341-344
Hauptverfasser: Hasumi, F, Fukuoka, K, Adachi, S, Miyamoto, Y, Okura, I
Format: Artikel
Sprache:eng
Schlagworte:
Alanine - chemical synthesis
Hydrogenase
Leucine - chemical synthesis
NAD
Oxidoreductases
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Zusammenfassung:Alanine synthesis by reductive amination of pyruvate was performed by the combination of NADH regeneration system and alanine dehydrogenase (AlaDH). The conversion of pyruvate to alanine was 99% after 1 h. Leucine synthesis was also carried out by the combination of NADH regeneration system and leucine dehydrogenase (LeuDH). The conversion of 4-methyl-2-oxovalerate to leucine was 60% after 1.5 h.
ISSN:0273-2289
1559-0291
DOI:10.1007/bf02786964