Isolation and characterisation of GLP-1 7–36 amide from rat intestine Elevated levels in diabetic rats
Glucagon-like peptide-1 (GLP-1) was purified to homogeneity by HPLC and anion-exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP-1 7–36 NH 2, providing evidence that amidation occurs at an arginine residue during the post-tr...
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| Veröffentlicht in: | FEBS letters 1988-12, Vol.242 (1), p.167-170 |
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| creator | Kreymann, B. Yiangou, Y. Kanse, S. Williams, G. Ghatei, M.A. Bloom, S.R. |
| description | Glucagon-like peptide-1 (GLP-1) was purified to homogeneity by HPLC and anion-exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP-1 7–36 NH
2, providing evidence that amidation occurs at an arginine residue during the post-translational processing of GLP-1. The distribution of GLP-1 7–36 NH
2-like immunoreactivity (GLP-1 7–36 NH
2 IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin-induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP-1 7–36 NH
2 IR in the colon. |
| doi_str_mv | 10.1016/0014-5793(88)81008-1 |
| format | Article |
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2, providing evidence that amidation occurs at an arginine residue during the post-translational processing of GLP-1. The distribution of GLP-1 7–36 NH
2-like immunoreactivity (GLP-1 7–36 NH
2 IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin-induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP-1 7–36 NH
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2, providing evidence that amidation occurs at an arginine residue during the post-translational processing of GLP-1. The distribution of GLP-1 7–36 NH
2-like immunoreactivity (GLP-1 7–36 NH
2 IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin-induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP-1 7–36 NH
2 IR in the colon.</description><subject>Animals</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Ion Exchange</subject><subject>Colon - analysis</subject><subject>Colon - metabolism</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Eating</subject><subject>Glucagon</subject><subject>Glucagon-Like Peptide 1</subject><subject>Glucagon-Like Peptides</subject><subject>Ileum - analysis</subject><subject>Ileum - metabolism</subject><subject>Intestinal Mucosa - metabolism</subject><subject>intestine</subject><subject>Intestines - analysis</subject><subject>Mass Spectrometry</subject><subject>Molecular Weight</subject><subject>Peptide Fragments</subject><subject>Peptide termination factor</subject><subject>Peptides - analysis</subject><subject>Peptides - isolation & purification</subject><subject>Peptides - metabolism</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Tissue Distribution</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1TAQhS0EKreFNyiSV4guUjx2Ek82SFDdWypdiS7K2nLsieoqP8XOLequ79A35ElImqsuKauRzzn-NDrD2DGIUxBQfhYC8qzQlfqEeIIgBGbwiq0AtcpUXuJrtnqOvGWHKd2I6Y1QHbADJYXSOazY9UUaWjuGoee299xd22jdSDGkRRwafr69zIDrPw-PquS2C554E4eORzvy0I-UxtATX7d0Z0fyfJrUpsnhPtiaxuDmZHrH3jS2TfR-P4_Yz8366ux7tv1xfnH2dZu5vADISqyhcD6vGo1KSCi0K0nX4CptFRRSOZQoG6WVl0qgbaCpNchSoxcWHaoj9nHh3sbh125aznQhOWpb29OwS0ZjgWWO8GIQCpCoqnwK5kvQxSGlSI25jaGz8d6AMPMlzFyzmWs2iObpEmbmf9jzd3VH_vnTvvrJ3yz-79DS_X8xzWb9Tc7GrCM-qTPoywKaaqe7QNEkF6h35EMkNxo_hH9v-hcdE6nN</recordid><startdate>19881219</startdate><enddate>19881219</enddate><creator>Kreymann, B.</creator><creator>Yiangou, Y.</creator><creator>Kanse, S.</creator><creator>Williams, G.</creator><creator>Ghatei, M.A.</creator><creator>Bloom, S.R.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19881219</creationdate><title>Isolation and characterisation of GLP-1 7–36 amide from rat intestine Elevated levels in diabetic rats</title><author>Kreymann, B. ; Yiangou, Y. ; Kanse, S. ; Williams, G. ; Ghatei, M.A. ; Bloom, S.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4511-68b15cd49f78302157c6e7b1c97a31523c8282f373d2308af1fb712678d0a8c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Ion Exchange</topic><topic>Colon - analysis</topic><topic>Colon - metabolism</topic><topic>Diabetes mellitus</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Eating</topic><topic>Glucagon</topic><topic>Glucagon-Like Peptide 1</topic><topic>Glucagon-Like Peptides</topic><topic>Ileum - analysis</topic><topic>Ileum - metabolism</topic><topic>Intestinal Mucosa - metabolism</topic><topic>intestine</topic><topic>Intestines - analysis</topic><topic>Mass Spectrometry</topic><topic>Molecular Weight</topic><topic>Peptide Fragments</topic><topic>Peptide termination factor</topic><topic>Peptides - analysis</topic><topic>Peptides - isolation & purification</topic><topic>Peptides - metabolism</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kreymann, B.</creatorcontrib><creatorcontrib>Yiangou, Y.</creatorcontrib><creatorcontrib>Kanse, S.</creatorcontrib><creatorcontrib>Williams, G.</creatorcontrib><creatorcontrib>Ghatei, M.A.</creatorcontrib><creatorcontrib>Bloom, S.R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kreymann, B.</au><au>Yiangou, Y.</au><au>Kanse, S.</au><au>Williams, G.</au><au>Ghatei, M.A.</au><au>Bloom, S.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterisation of GLP-1 7–36 amide from rat intestine Elevated levels in diabetic rats</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1988-12-19</date><risdate>1988</risdate><volume>242</volume><issue>1</issue><spage>167</spage><epage>170</epage><pages>167-170</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Glucagon-like peptide-1 (GLP-1) was purified to homogeneity by HPLC and anion-exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP-1 7–36 NH
2, providing evidence that amidation occurs at an arginine residue during the post-translational processing of GLP-1. The distribution of GLP-1 7–36 NH
2-like immunoreactivity (GLP-1 7–36 NH
2 IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin-induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP-1 7–36 NH
2 IR in the colon.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>3203741</pmid><doi>10.1016/0014-5793(88)81008-1</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Chromatography Chromatography, High Pressure Liquid Chromatography, Ion Exchange Colon - analysis Colon - metabolism Diabetes mellitus Diabetes Mellitus, Experimental - metabolism Eating Glucagon Glucagon-Like Peptide 1 Glucagon-Like Peptides Ileum - analysis Ileum - metabolism Intestinal Mucosa - metabolism intestine Intestines - analysis Mass Spectrometry Molecular Weight Peptide Fragments Peptide termination factor Peptides - analysis Peptides - isolation & purification Peptides - metabolism Rat Rats Rats, Inbred Strains Tissue Distribution |
| title | Isolation and characterisation of GLP-1 7–36 amide from rat intestine Elevated levels in diabetic rats |
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