Isolation and characterisation of GLP-1 7–36 amide from rat intestine Elevated levels in diabetic rats

Isolation and characterisation of GLP-1 7–36 amide from rat intestine Elevated levels in diabetic rats

Glucagon-like peptide-1 (GLP-1) was purified to homogeneity by HPLC and anion-exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP-1 7–36 NH 2, providing evidence that amidation occurs at an arginine residue during the post-tr...

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Veröffentlicht in:FEBS letters 1988-12, Vol.242 (1), p.167-170
Hauptverfasser: Kreymann, B., Yiangou, Y., Kanse, S., Williams, G., Ghatei, M.A., Bloom, S.R.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Chromatography
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Colon - analysis
Colon - metabolism
Diabetes mellitus
Diabetes Mellitus, Experimental - metabolism
Eating
Glucagon
Glucagon-Like Peptide 1
Glucagon-Like Peptides
Ileum - analysis
Ileum - metabolism
Intestinal Mucosa - metabolism
intestine
Intestines - analysis
Mass Spectrometry
Molecular Weight
Peptide Fragments
Peptide termination factor
Peptides - analysis
Peptides - isolation & purification
Peptides - metabolism
Rat
Rats
Rats, Inbred Strains
Tissue Distribution
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Zusammenfassung:Glucagon-like peptide-1 (GLP-1) was purified to homogeneity by HPLC and anion-exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP-1 7–36 NH 2, providing evidence that amidation occurs at an arginine residue during the post-translational processing of GLP-1. The distribution of GLP-1 7–36 NH 2-like immunoreactivity (GLP-1 7–36 NH 2 IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin-induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP-1 7–36 NH 2 IR in the colon.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(88)81008-1