The nPCR detection of Chlamydia pneumoniae and Chlamydia trachomatis in children hospitalized for bronchiolitis

Nasopharyngeal aspirates from children admitted with the diagnosis of bronchiolitis, were screened for Chlamydia pneumoniae, and C. trachomatis. The nested PCR was found to be more sensitive that amplified the DNA extracts of up to two elementary bodies of the test strains. Using this technique, C....

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Veröffentlicht in:	The Journal of infection 1996-11, Vol.33 (3), p.173-175
Hauptverfasser:	Khan, M.A., Potter, C.W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Bacterial - analysis Bacterial diseases Bacterial diseases of the respiratory system Biological and medical sciences Bronchiolitis - microbiology Child, Preschool Chlamydia Infections - microbiology Chlamydia pneumoniae Chlamydia trachomatis Chlamydia trachomatis - isolation & purification Chlamydophila pneumoniae - isolation & purification DNA, Bacterial - analysis Electrophoresis, Agar Gel Human bacterial diseases Humans Infant Infant, Newborn Infectious diseases Medical sciences Molecular Sequence Data Polymerase Chain Reaction Sensitivity and Specificity
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Zusammenfassung:	Nasopharyngeal aspirates from children admitted with the diagnosis of bronchiolitis, were screened for Chlamydia pneumoniae, and C. trachomatis. The nested PCR was found to be more sensitive that amplified the DNA extracts of up to two elementary bodies of the test strains. Using this technique, C. pneumoniae was detected in two (1.3%), and C. trachomatis in 26 (17.1%) of the 152 samples tested. This study indicated C. pneumoniae infections to be unusual in children with bronchiolitis.
ISSN:	0163-4453 1532-2742
DOI:	10.1016/S0163-4453(96)92177-5