Flagellin parts acquiring a regular structure during polymerization are disposed on the molecule ends

Flagellins of two Escherichia coli strains have been investigated by limited proteolysis and scanning microcalorimetry. It has been shown that a monomer flagellin consists of two parts: a central one cooperatively melting, rather resistant to proteases, and the other without a stable tertiary struct...

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Veröffentlicht in:	FEBS letters 1988-12, Vol.241 (1), p.141-144
Hauptverfasser:	Kostyukova, A.S., Pyatibratov, M.G., Filimonov, V.V., Fedorov, O.V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Bacterial Proteins - metabolism Biological and medical sciences Chymotrypsin Cyanogen Bromide Escherichia coli Escherichia coli - metabolism Flagellin Flagellin - metabolism Fundamental and applied biological sciences. Psychology Holoproteins Limited proteolysis Macromolecular Substances Microcalorimetry Molecular Weight Other proteins PAGE, polyacrylamide gel electrophoresis Peptide Fragments - analysis Proteins SDS, sodium dodecyl sulfate Thermodynamics Thermolysin Trypsin
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Beschreibung
Zusammenfassung:	Flagellins of two Escherichia coli strains have been investigated by limited proteolysis and scanning microcalorimetry. It has been shown that a monomer flagellin consists of two parts: a central one cooperatively melting, rather resistant to proteases, and the other without a stable tertiary structure and thus easily degrading terminals. Just these terminals acquire a regular structure during polymerization. Core fragments of the two strains have been isolated and characterized.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(88)81047-0