Sequencing proteins from acrylamide gels

Sequencing proteins from acrylamide gels

The analytical power of high-resolution two-dimensional gel electrophoresis has been coupled with molecular cloning techniques to allow the sequencing of proteins directly from preparative gels.

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Bibliographische Detailangaben
Veröffentlicht in:Nature (London) 1988-12, Vol.336 (6198), p.499-500
Hauptverfasser: Kennedy, T. E, Wager-Smith, K, Barzila, A, Kandel, E. R, Sweatt, J. D
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Animals
Aplysia - analysis
Cloning
Cloning, Molecular
DNA - genetics
DNA Probes
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Gels
Molecular Sequence Data
Molecules
Peptide Fragments - isolation & purification
Proteins
Proteins - genetics
Proteins - isolation & purification
Proteins - metabolism
Serine Endopeptidases - metabolism
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Beschreibung
Zusammenfassung:The analytical power of high-resolution two-dimensional gel electrophoresis has been coupled with molecular cloning techniques to allow the sequencing of proteins directly from preparative gels.
ISSN:0028-0836
1476-4687
DOI:10.1038/336499a0