Age and sex related changes of plasma membrane fluidity in isolated rat hepatocytes

The influence of sex and age on membrane fluidity, has been investigated in 6, 12, 18 weeks old Sprague-Dawley rats. Fluorescence polarization (P) was determined at 37°C with a Perkin Elmer MPF 44A fluorescence spectrophotometer. The fluorescent probe TMA-DPH was added to isolated hepato cytes prepa...

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Veröffentlicht in:	Biochemical and biophysical research communications 1988-10, Vol.156 (2), p.840-845
Hauptverfasser:	Benedetti, Antonio, Ferretti, Gianna, Curatola, Giovanna, Jézéquel, Anne Marie, Orlandi, Francesco
Format:	Artikel
Sprache:	eng
Schlagworte:	Aging - physiology Animals Biological and medical sciences Cell Membrane - physiology Cell membranes. Ionic channels. Membrane pores Cell structures and functions Diphenylhexatriene - analogs & derivatives Female Fluorescence Polarization Fluorescent Dyes Fundamental and applied biological sciences. Psychology Liver - ultrastructure Male Membrane Fluidity Molecular and cellular biology Rats Rats, Inbred Strains Sex Characteristics
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Zusammenfassung:	The influence of sex and age on membrane fluidity, has been investigated in 6, 12, 18 weeks old Sprague-Dawley rats. Fluorescence polarization (P) was determined at 37°C with a Perkin Elmer MPF 44A fluorescence spectrophotometer. The fluorescent probe TMA-DPH was added to isolated hepato cytes prepared by collagenase method. The membrane fluidity was constantly lower in males than in females, but the difference was statistically significant only in the 12 weeks old group. Major differences appeared related to aging with a significant age-related decrease in fluidity in all animals.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/S0006-291X(88)80920-3