Genomic Organization and DNA Sequences of Two Human Phenol Sulfotransferase Genes (STP1andSTP2) on the Short Arm of Chromosome 16

A family of human phenol sulfotransferase genes has been suggested by the cloning of numerous cDNA isolates from different tissues. We have previously cloned and sequenced theSTMgene encoding the monoamine neurotransmitter-preferring sulfotransferase, M-PST, and a portion of theSTP1gene encoding the phenol-preferring isozyme, P-PST1 (BBRC205,1325–1332;Genomics18,440–443). Both genes were mapped to a small region on the short arm of chromosome 16 (BBRC205,482–489). Here we report on the sequencing and genomic organization of theSTP1andSTP2genes from a single cosmid clone obtained from chromosome 16p12.1-p11.2.STP1andSTP2are 95.9% identical at the amino acid sequence level, whereas theSTMgene is only 92.9% and 90.5% identical toSTP1andSTP2,respectively. Alignment of the genomic sequences indicated that all three genes have 7 coding exons and conserved intron-exon boundaries. These results facilitated the assignment of previously published cDNA isolates as “alleles” of the individualSTM, STP1,andSTP2loci on 16p, and provide to us a greater understanding of the complexity and roles of the phenol sulfotransferase gene family in the metabolism of endogenous and xenobiotic agents.