Transcriptional Regulation of the N-Acetylglucosaminyltransferase V Gene in Human Bile Duct Carcinoma Cells (HuCC-T1) Is Mediated by Ets-1
N -Acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of N -acetylglucosamine from UDP- N -acetylglucosamine to α-6- D -mannoside to produce the β1-6 linked branching of N -glycan oligosaccharides, which controls the polylactosamine content. The expression of N -acetylglucosaminyltrans...
Gespeichert in:
Veröffentlicht in: | The Journal of biological chemistry 1996-10, Vol.271 (43), p.26706-26712 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | N -Acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of N -acetylglucosamine from UDP- N -acetylglucosamine to α-6- D -mannoside to produce the β1-6 linked branching of N -glycan oligosaccharides, which controls the polylactosamine content. The expression of N -acetylglucosaminyltransferase V, which contains 17 exons and spans 155 kilobase pairs, is expressed in a tissue- and cell
type-specific manner and is regulated at the level of transcription by multiple promoters (Saito, H., Gu, J., Nishikawa, A.,
Ihara, Y., Fujii, J., Kohgo, Y., and Taniguchi, N. (1995) Eur. J. Biochem. 233, 18-26). To elucidate the mechanism by which the GnT-V gene is expressed in a cell- and tissue-specific manner, cell-restricted
expression was analyzed using the 5â²-upstream regions of the human GnT-V gene spanning base pairs â2760 to +23 in a human
bile duct carcinoma cell line, HuCC-T1. We characterized two cis -acting elements that are potentially important in HuCC-T1 cell-specific expression. The two elements each contain an Ets-1
binding site, 5â²-GGA-3â². Specific binding of Ets-1 to the respective elements was demonstrated by competition analysis as
well as by antibody supershift experiments. Cotransfection of an Ets-1 expression plasmid along with a GnT-V promoter-luciferase
reporter plasmid revealed the participation of Ets-1 in the regulation of the GnT-V gene transcription. These data indicated
that the transcriptional regulation of the GnT-V gene was mediated by transcription factor Ets-1. |
---|---|
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.271.43.26706 |