Evaluation of a reverse transcriptase-polymerase chain reaction assay for detecting St. Louis encephalitis virus using field-collected mosquitoes (Diptera: Culicidae)
A reverse transcriptase-polymerase chain reaction assay (RT-PCR) was compared with a tissue culture assay (TC) and an enzyme immunoassay (EIA) to detect St. Louis encephalitis virus (SLEV) in mosquito pools. Overall, 1,725 mosquito pools with a low viral prevalence (3.3%-5.0%) were tested. The compa...
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| Veröffentlicht in: | Journal of medical entomology 1996, Vol.33 (1), p.123-127 |
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| container_title | Journal of medical entomology |
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| creator | NAWROCKI, S. J RANDLE, Y. H VODKIN, M. H SIEGEL, J. P NOVAK, R. J |
| description | A reverse transcriptase-polymerase chain reaction assay (RT-PCR) was compared with a tissue culture assay (TC) and an enzyme immunoassay (EIA) to detect St. Louis encephalitis virus (SLEV) in mosquito pools. Overall, 1,725 mosquito pools with a low viral prevalence (3.3%-5.0%) were tested. The comparison of the EIA to TC showed that the EIA was 0.947 sensitive, 0.988 specific, and 0.987 accurate. Comparison of RT-PCR to TC showed that the RT-PCR was 0.947 sensitive, 0.980 specific, and 0.979 accurate. Comparison of the RT-PCR to EIA showed that the RT-PCR was 0.878 sensitive, 0.987 specific, and 0.982 accurate. Because of speed, accuracy, and cost, either the RT-PCR or the EIA is recommended as the primary screen. RT-PCR has an advantage over EIA, because the amplified product contains sequence information which can confirm its identity. TC, EIA, or both can be applied as a 2nd assay for the confirmation of samples suspected as positives by the RT-PCR. |
| doi_str_mv | 10.1093/jmedent/33.1.123 |
| format | Article |
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J ; RANDLE, Y. H ; VODKIN, M. H ; SIEGEL, J. P ; NOVAK, R. J</creator><creatorcontrib>NAWROCKI, S. J ; RANDLE, Y. H ; VODKIN, M. H ; SIEGEL, J. P ; NOVAK, R. J ; Nezavisima Fondatsiya "Veterinarna Meditsina", Sofia (Bulgaria) ; Harris County Mosquito Control District, Houston, TX</creatorcontrib><description>A reverse transcriptase-polymerase chain reaction assay (RT-PCR) was compared with a tissue culture assay (TC) and an enzyme immunoassay (EIA) to detect St. Louis encephalitis virus (SLEV) in mosquito pools. Overall, 1,725 mosquito pools with a low viral prevalence (3.3%-5.0%) were tested. The comparison of the EIA to TC showed that the EIA was 0.947 sensitive, 0.988 specific, and 0.987 accurate. Comparison of RT-PCR to TC showed that the RT-PCR was 0.947 sensitive, 0.980 specific, and 0.979 accurate. Comparison of the RT-PCR to EIA showed that the RT-PCR was 0.878 sensitive, 0.987 specific, and 0.982 accurate. Because of speed, accuracy, and cost, either the RT-PCR or the EIA is recommended as the primary screen. RT-PCR has an advantage over EIA, because the amplified product contains sequence information which can confirm its identity. TC, EIA, or both can be applied as a 2nd assay for the confirmation of samples suspected as positives by the RT-PCR.</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 1938-2928</identifier><identifier>DOI: 10.1093/jmedent/33.1.123</identifier><identifier>PMID: 8906915</identifier><identifier>CODEN: JMENA6</identifier><language>eng</language><publisher>Lanham, MD: Entomological Society of America</publisher><subject>analisis de costos ; analyse des couts ; Animals ; Base Sequence ; Biological and medical sciences ; cost analysis ; Culex pipiens ; culex quinquefasciatus ; Culicidae ; Culicidae - virology ; cultivo de tejidos ; culture de tissu ; Culture Techniques ; Diptera ; DNA Primers ; Encephalitis Virus, St. Louis - genetics ; Encephalitis Virus, St. Louis - isolation & purification ; Evaluation Studies as Topic ; experimentacion ; experimentation ; Female ; flavivirus ; Fundamental and applied biological sciences. Psychology ; Immunoenzyme Techniques ; Male ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; Molecular Sequence Data ; pcr ; Polymerase Chain Reaction - methods ; reverse transcriptase ; RNA-Directed DNA Polymerase ; Sensitivity and Specificity ; St. Louis encephalitis virus ; technique immunoenzymatique ; tecnicas inmunoenzimaticas ; texas ; tissue culture ; transcriptasa inversa ; transcriptase inverse ; vecteur de maladie ; vectores ; vectors ; Vectors. Intermediate hosts</subject><ispartof>Journal of medical entomology, 1996, Vol.33 (1), p.123-127</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-677f086181784d8a80c198cdeb4f41f79acc13eada93dc2738662d415b6929a33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3030838$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8906915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NAWROCKI, S. J</creatorcontrib><creatorcontrib>RANDLE, Y. H</creatorcontrib><creatorcontrib>VODKIN, M. H</creatorcontrib><creatorcontrib>SIEGEL, J. P</creatorcontrib><creatorcontrib>NOVAK, R. J</creatorcontrib><creatorcontrib>Nezavisima Fondatsiya "Veterinarna Meditsina", Sofia (Bulgaria)</creatorcontrib><creatorcontrib>Harris County Mosquito Control District, Houston, TX</creatorcontrib><title>Evaluation of a reverse transcriptase-polymerase chain reaction assay for detecting St. Louis encephalitis virus using field-collected mosquitoes (Diptera: Culicidae)</title><title>Journal of medical entomology</title><addtitle>J Med Entomol</addtitle><description>A reverse transcriptase-polymerase chain reaction assay (RT-PCR) was compared with a tissue culture assay (TC) and an enzyme immunoassay (EIA) to detect St. Louis encephalitis virus (SLEV) in mosquito pools. Overall, 1,725 mosquito pools with a low viral prevalence (3.3%-5.0%) were tested. The comparison of the EIA to TC showed that the EIA was 0.947 sensitive, 0.988 specific, and 0.987 accurate. Comparison of RT-PCR to TC showed that the RT-PCR was 0.947 sensitive, 0.980 specific, and 0.979 accurate. Comparison of the RT-PCR to EIA showed that the RT-PCR was 0.878 sensitive, 0.987 specific, and 0.982 accurate. Because of speed, accuracy, and cost, either the RT-PCR or the EIA is recommended as the primary screen. RT-PCR has an advantage over EIA, because the amplified product contains sequence information which can confirm its identity. TC, EIA, or both can be applied as a 2nd assay for the confirmation of samples suspected as positives by the RT-PCR.</description><subject>analisis de costos</subject><subject>analyse des couts</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cost analysis</subject><subject>Culex pipiens</subject><subject>culex quinquefasciatus</subject><subject>Culicidae</subject><subject>Culicidae - virology</subject><subject>cultivo de tejidos</subject><subject>culture de tissu</subject><subject>Culture Techniques</subject><subject>Diptera</subject><subject>DNA Primers</subject><subject>Encephalitis Virus, St. Louis - genetics</subject><subject>Encephalitis Virus, St. Louis - isolation & purification</subject><subject>Evaluation Studies as Topic</subject><subject>experimentacion</subject><subject>experimentation</subject><subject>Female</subject><subject>flavivirus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoenzyme Techniques</subject><subject>Male</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>Molecular Sequence Data</subject><subject>pcr</subject><subject>Polymerase Chain Reaction - methods</subject><subject>reverse transcriptase</subject><subject>RNA-Directed DNA Polymerase</subject><subject>Sensitivity and Specificity</subject><subject>St. Louis encephalitis virus</subject><subject>technique immunoenzymatique</subject><subject>tecnicas inmunoenzimaticas</subject><subject>texas</subject><subject>tissue culture</subject><subject>transcriptasa inversa</subject><subject>transcriptase inverse</subject><subject>vecteur de maladie</subject><subject>vectores</subject><subject>vectors</subject><subject>Vectors. Intermediate hosts</subject><issn>0022-2585</issn><issn>1938-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhiMEKkvhzgXkA0JwyNaOE3_0hpbyIa3EofQczdrj1pUTb-1kpf1D_E5cNuqVk8fzPu_M4Z2qesvomlHNL-4HtDhOF5yv2Zo1_Fm1YpqrutGNel6tKG2auulU97J6lfM9pVSxVp9VZ0pToVm3qv5cHSDMMPk4kugIkIQHTBnJlGDMJvn9BBnrfQzHAVMpibkDPxYMzD8T5AxH4mIiFicsvfGWXE9rso2zzwRHg_s7CH4qn4NPcyZzfkScx2BrE0MoHrRkiPlh9lPETD59LUvLrkuymYM33gJ-fl29cBAyvlne8-rm29XvzY96--v7z82XbW24lFMtpHRUCaaYVK1VoKhhWhmLu9a1zEkNxjCOYEFzaxrJlRCNbVm3E7rRwPl59fE0d5_iw4x56gefDYYAI8Y591J1rNNS_xdkkorCigLSE2hSzDmh6_fJD5COPaP9Y4b9kmHPec_6kmGxvFtmz7uiPRmW0Ir-YdEhGwiuJGV8fsI45VRxVbD3J8xB7OE2FeTmmmkty1W0rRD8L7WossI</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>NAWROCKI, S. J</creator><creator>RANDLE, Y. H</creator><creator>VODKIN, M. H</creator><creator>SIEGEL, J. P</creator><creator>NOVAK, R. 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J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-677f086181784d8a80c198cdeb4f41f79acc13eada93dc2738662d415b6929a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>analisis de costos</topic><topic>analyse des couts</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cost analysis</topic><topic>Culex pipiens</topic><topic>culex quinquefasciatus</topic><topic>Culicidae</topic><topic>Culicidae - virology</topic><topic>cultivo de tejidos</topic><topic>culture de tissu</topic><topic>Culture Techniques</topic><topic>Diptera</topic><topic>DNA Primers</topic><topic>Encephalitis Virus, St. Louis - genetics</topic><topic>Encephalitis Virus, St. Louis - isolation & purification</topic><topic>Evaluation Studies as Topic</topic><topic>experimentacion</topic><topic>experimentation</topic><topic>Female</topic><topic>flavivirus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoenzyme Techniques</topic><topic>Male</topic><topic>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</topic><topic>Molecular Sequence Data</topic><topic>pcr</topic><topic>Polymerase Chain Reaction - methods</topic><topic>reverse transcriptase</topic><topic>RNA-Directed DNA Polymerase</topic><topic>Sensitivity and Specificity</topic><topic>St. Louis encephalitis virus</topic><topic>technique immunoenzymatique</topic><topic>tecnicas inmunoenzimaticas</topic><topic>texas</topic><topic>tissue culture</topic><topic>transcriptasa inversa</topic><topic>transcriptase inverse</topic><topic>vecteur de maladie</topic><topic>vectores</topic><topic>vectors</topic><topic>Vectors. Intermediate hosts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NAWROCKI, S. J</creatorcontrib><creatorcontrib>RANDLE, Y. H</creatorcontrib><creatorcontrib>VODKIN, M. H</creatorcontrib><creatorcontrib>SIEGEL, J. P</creatorcontrib><creatorcontrib>NOVAK, R. J</creatorcontrib><creatorcontrib>Nezavisima Fondatsiya "Veterinarna Meditsina", Sofia (Bulgaria)</creatorcontrib><creatorcontrib>Harris County Mosquito Control District, Houston, TX</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NAWROCKI, S. J</au><au>RANDLE, Y. H</au><au>VODKIN, M. H</au><au>SIEGEL, J. P</au><au>NOVAK, R. J</au><aucorp>Nezavisima Fondatsiya "Veterinarna Meditsina", Sofia (Bulgaria)</aucorp><aucorp>Harris County Mosquito Control District, Houston, TX</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a reverse transcriptase-polymerase chain reaction assay for detecting St. Louis encephalitis virus using field-collected mosquitoes (Diptera: Culicidae)</atitle><jtitle>Journal of medical entomology</jtitle><addtitle>J Med Entomol</addtitle><date>1996</date><risdate>1996</risdate><volume>33</volume><issue>1</issue><spage>123</spage><epage>127</epage><pages>123-127</pages><issn>0022-2585</issn><eissn>1938-2928</eissn><coden>JMENA6</coden><abstract>A reverse transcriptase-polymerase chain reaction assay (RT-PCR) was compared with a tissue culture assay (TC) and an enzyme immunoassay (EIA) to detect St. Louis encephalitis virus (SLEV) in mosquito pools. Overall, 1,725 mosquito pools with a low viral prevalence (3.3%-5.0%) were tested. The comparison of the EIA to TC showed that the EIA was 0.947 sensitive, 0.988 specific, and 0.987 accurate. Comparison of RT-PCR to TC showed that the RT-PCR was 0.947 sensitive, 0.980 specific, and 0.979 accurate. Comparison of the RT-PCR to EIA showed that the RT-PCR was 0.878 sensitive, 0.987 specific, and 0.982 accurate. Because of speed, accuracy, and cost, either the RT-PCR or the EIA is recommended as the primary screen. RT-PCR has an advantage over EIA, because the amplified product contains sequence information which can confirm its identity. TC, EIA, or both can be applied as a 2nd assay for the confirmation of samples suspected as positives by the RT-PCR.</abstract><cop>Lanham, MD</cop><pub>Entomological Society of America</pub><pmid>8906915</pmid><doi>10.1093/jmedent/33.1.123</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2585 |
| ispartof | Journal of medical entomology, 1996, Vol.33 (1), p.123-127 |
| issn | 0022-2585 1938-2928 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | analisis de costos analyse des couts Animals Base Sequence Biological and medical sciences cost analysis Culex pipiens culex quinquefasciatus Culicidae Culicidae - virology cultivo de tejidos culture de tissu Culture Techniques Diptera DNA Primers Encephalitis Virus, St. Louis - genetics Encephalitis Virus, St. Louis - isolation & purification Evaluation Studies as Topic experimentacion experimentation Female flavivirus Fundamental and applied biological sciences. Psychology Immunoenzyme Techniques Male Medically important nuisances and vectors, pests of stored products and materials: population survey and control Molecular Sequence Data pcr Polymerase Chain Reaction - methods reverse transcriptase RNA-Directed DNA Polymerase Sensitivity and Specificity St. Louis encephalitis virus technique immunoenzymatique tecnicas inmunoenzimaticas texas tissue culture transcriptasa inversa transcriptase inverse vecteur de maladie vectores vectors Vectors. Intermediate hosts |
| title | Evaluation of a reverse transcriptase-polymerase chain reaction assay for detecting St. Louis encephalitis virus using field-collected mosquitoes (Diptera: Culicidae) |
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