Assessment of anti-HLA antibodies in sera being tested for platelet reactivity by a platelet lymphocyte immunofluorescence test (PLIFT)

An indirect immunofluorescence test using a platelet mononuclear cell suspension is described. The test is a simple and sensitive method to assess the contamination by anti-HLA antibodies of sera being tested for platelet reactivity and eliminates the need to support two independent test systems suc...

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Veröffentlicht in:Journal of immunological methods 1988-11, Vol.114 (1), p.187-190
Hauptverfasser: Muylle, L., Van Bockstaele, D., De Smet, D., Peetermans, M.E.
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container_end_page 190
container_issue 1
container_start_page 187
container_title Journal of immunological methods
container_volume 114
creator Muylle, L.
Van Bockstaele, D.
De Smet, D.
Peetermans, M.E.
description An indirect immunofluorescence test using a platelet mononuclear cell suspension is described. The test is a simple and sensitive method to assess the contamination by anti-HLA antibodies of sera being tested for platelet reactivity and eliminates the need to support two independent test systems such as lymphocytotoxicity and immunofluorescence testing. The test could be of value in routine platelet serology testing and platelet crossmatching.
doi_str_mv 10.1016/0022-1759(88)90172-X
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source Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE
subjects Antigen-Antibody Reactions
Antilymphocyte Serum - analysis
Biological and medical sciences
Blood Platelets - analysis
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Histocompatibility Testing - methods
HLA antibody
HLA Antigens - immunology
Humans
Immune Sera - analysis
Immunofluorescence test
Isoantibodies - analysis
Lymphocytes - analysis
Platelet antibody
Suspensions
title Assessment of anti-HLA antibodies in sera being tested for platelet reactivity by a platelet lymphocyte immunofluorescence test (PLIFT)
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