Assessment of anti-HLA antibodies in sera being tested for platelet reactivity by a platelet lymphocyte immunofluorescence test (PLIFT)
An indirect immunofluorescence test using a platelet mononuclear cell suspension is described. The test is a simple and sensitive method to assess the contamination by anti-HLA antibodies of sera being tested for platelet reactivity and eliminates the need to support two independent test systems suc...
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Veröffentlicht in: | Journal of immunological methods 1988-11, Vol.114 (1), p.187-190 |
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container_title | Journal of immunological methods |
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creator | Muylle, L. Van Bockstaele, D. De Smet, D. Peetermans, M.E. |
description | An indirect immunofluorescence test using a platelet mononuclear cell suspension is described. The test is a simple and sensitive method to assess the contamination by anti-HLA antibodies of sera being tested for platelet reactivity and eliminates the need to support two independent test systems such as lymphocytotoxicity and immunofluorescence testing. The test could be of value in routine platelet serology testing and platelet crossmatching. |
doi_str_mv | 10.1016/0022-1759(88)90172-X |
format | Article |
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The test is a simple and sensitive method to assess the contamination by anti-HLA antibodies of sera being tested for platelet reactivity and eliminates the need to support two independent test systems such as lymphocytotoxicity and immunofluorescence testing. The test could be of value in routine platelet serology testing and platelet crossmatching.</description><subject>Antigen-Antibody Reactions</subject><subject>Antilymphocyte Serum - analysis</subject><subject>Biological and medical sciences</subject><subject>Blood Platelets - analysis</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Histocompatibility Testing - methods</subject><subject>HLA antibody</subject><subject>HLA Antigens - immunology</subject><subject>Humans</subject><subject>Immune Sera - analysis</subject><subject>Immunofluorescence test</subject><subject>Isoantibodies - analysis</subject><subject>Lymphocytes - analysis</subject><subject>Platelet antibody</subject><subject>Suspensions</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1q3TAQhUVpSW_SvkEL2rQkC7cjW7LlTeASmh-40C5SyE7I0rhVsa1bSQ74Cfra1f0h2bWrGZjvHA5zCHnH4BMDVn8GKMuCNaI9l_KiBdaUxcMLsmIyL00L4iVZPSGvyWmMvwCAQQ0n5KQCUbXQrsifdYwY44hTor6nekquuN2s90vnrcNI3UQjBk07dNMPmjAmtLT3gW4HnXDARANqk9yjSwvtFqqfD8Mybn96sySkbhznyffD7ANGg5PBvRU9_7a5u76_eENe9XqI-PY4z8j36y_3V7fF5uvN3dV6UxjOZCo0ILdoG8m5bq2xndCo-xJ1zTUaIYxhxlQcK962ZUZ4x0RlOxCil9aWvDojHw--2-B_zzmAGl2OMwx6Qj9H1UgBFWTR_0AmGFR1WWeQH0ATfIwBe7UNbtRhUQzUrii1a0HtWlBSqn1R6iHL3h_9525E-yQ6NpPvH453HY0e-qAn4-KzdytECRwyd3ngMH_t0WFQ0bjde60LaJKy3v07yF-Gp7HN</recordid><startdate>19881110</startdate><enddate>19881110</enddate><creator>Muylle, L.</creator><creator>Van Bockstaele, D.</creator><creator>De Smet, D.</creator><creator>Peetermans, M.E.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19881110</creationdate><title>Assessment of anti-HLA antibodies in sera being tested for platelet reactivity by a platelet lymphocyte immunofluorescence test (PLIFT)</title><author>Muylle, L. ; Van Bockstaele, D. ; De Smet, D. ; Peetermans, M.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-a0e4ded7844a9dcdb5aeaf2ea64aec55cc1cc34e349924a94b153db055f8dd243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Antigen-Antibody Reactions</topic><topic>Antilymphocyte Serum - analysis</topic><topic>Biological and medical sciences</topic><topic>Blood Platelets - analysis</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Histocompatibility Testing - methods</topic><topic>HLA antibody</topic><topic>HLA Antigens - immunology</topic><topic>Humans</topic><topic>Immune Sera - analysis</topic><topic>Immunofluorescence test</topic><topic>Isoantibodies - analysis</topic><topic>Lymphocytes - analysis</topic><topic>Platelet antibody</topic><topic>Suspensions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muylle, L.</creatorcontrib><creatorcontrib>Van Bockstaele, D.</creatorcontrib><creatorcontrib>De Smet, D.</creatorcontrib><creatorcontrib>Peetermans, M.E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muylle, L.</au><au>Van Bockstaele, D.</au><au>De Smet, D.</au><au>Peetermans, M.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of anti-HLA antibodies in sera being tested for platelet reactivity by a platelet lymphocyte immunofluorescence test (PLIFT)</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1988-11-10</date><risdate>1988</risdate><volume>114</volume><issue>1</issue><spage>187</spage><epage>190</epage><pages>187-190</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>An indirect immunofluorescence test using a platelet mononuclear cell suspension is described. The test is a simple and sensitive method to assess the contamination by anti-HLA antibodies of sera being tested for platelet reactivity and eliminates the need to support two independent test systems such as lymphocytotoxicity and immunofluorescence testing. The test could be of value in routine platelet serology testing and platelet crossmatching.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3053909</pmid><doi>10.1016/0022-1759(88)90172-X</doi><tpages>4</tpages></addata></record> |
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subjects | Antigen-Antibody Reactions Antilymphocyte Serum - analysis Biological and medical sciences Blood Platelets - analysis Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Fundamental immunology Histocompatibility Testing - methods HLA antibody HLA Antigens - immunology Humans Immune Sera - analysis Immunofluorescence test Isoantibodies - analysis Lymphocytes - analysis Platelet antibody Suspensions |
title | Assessment of anti-HLA antibodies in sera being tested for platelet reactivity by a platelet lymphocyte immunofluorescence test (PLIFT) |
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