Assessment of anti-HLA antibodies in sera being tested for platelet reactivity by a platelet lymphocyte immunofluorescence test (PLIFT)

An indirect immunofluorescence test using a platelet mononuclear cell suspension is described. The test is a simple and sensitive method to assess the contamination by anti-HLA antibodies of sera being tested for platelet reactivity and eliminates the need to support two independent test systems suc...

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Veröffentlicht in:	Journal of immunological methods 1988-11, Vol.114 (1), p.187-190
Hauptverfasser:	Muylle, L., Van Bockstaele, D., De Smet, D., Peetermans, M.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigen-Antibody Reactions Antilymphocyte Serum - analysis Biological and medical sciences Blood Platelets - analysis Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Fundamental immunology Histocompatibility Testing - methods HLA antibody HLA Antigens - immunology Humans Immune Sera - analysis Immunofluorescence test Isoantibodies - analysis Lymphocytes - analysis Platelet antibody Suspensions
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Beschreibung
Zusammenfassung:	An indirect immunofluorescence test using a platelet mononuclear cell suspension is described. The test is a simple and sensitive method to assess the contamination by anti-HLA antibodies of sera being tested for platelet reactivity and eliminates the need to support two independent test systems such as lymphocytotoxicity and immunofluorescence testing. The test could be of value in routine platelet serology testing and platelet crossmatching.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(88)90172-X