Simple procedure for estimating the efficiency of PCR

The extension efficiency of PCR-driven DNA amplification was examined by step-controlled limiting dilution method using as internal controls 8E5 lymphocytes carrying a single copy of HIV genome. The results reveal that under standard conditions recommended by the PCR kit manufacturer the exponential...

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Veröffentlicht in:	Molecular biotechnology 1996-08, Vol.6 (1), p.87-89
Hauptverfasser:	BOURINBAIAR, A. S, AJUANG-SIMBIRI, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Diverse techniques DNA - chemical synthesis Fundamental and applied biological sciences. Psychology Molecular and cellular biology Polymerase Chain Reaction - methods
Online-Zugang:	Volltext
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