Simple procedure for estimating the efficiency of PCR

Simple procedure for estimating the efficiency of PCR

The extension efficiency of PCR-driven DNA amplification was examined by step-controlled limiting dilution method using as internal controls 8E5 lymphocytes carrying a single copy of HIV genome. The results reveal that under standard conditions recommended by the PCR kit manufacturer the exponential...

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Veröffentlicht in:Molecular biotechnology 1996-08, Vol.6 (1), p.87-89
Hauptverfasser: BOURINBAIAR, A. S, AJUANG-SIMBIRI, K
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Diverse techniques
DNA - chemical synthesis
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Polymerase Chain Reaction - methods
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Zusammenfassung:The extension efficiency of PCR-driven DNA amplification was examined by step-controlled limiting dilution method using as internal controls 8E5 lymphocytes carrying a single copy of HIV genome. The results reveal that under standard conditions recommended by the PCR kit manufacturer the exponential growth of DNA template is approximately one third of the theoretical doubling rate.
ISSN:1073-6085
1559-0305
DOI:10.1007/BF02762328