Immunosuppression by Anti-ICAM-1 and Anti-LFA-1 Monoclonal Antibodies of Free and Vascularized Skin Allograft Rejection

Immunosuppression by anti-adhesion molecule antibody of free or vascularized skin allograft rejection was investigated in rats. Lewis (LEW, RT1 1) rats were used as donors and Fisher (F344, RT1 1v1) rats as the recipients. When F344 rats were treated intraperitoneally (i.p.) with anti-intercellular adhesion molecule-1 (ICAM-1) mAb (1A29) (3 mg/kg/day) and anti-leukocyte function-associated antigen,-1 (LFA-1) mAb (WT. 1) (3 mg/kg/day) one day prior to grafting and daily after grafting for nine days, free skin graft survival was prolonged only slightly compared with that in control rats which were injected i.p. with a daily dose of 6 mg/kg of anti-TNP mAbs (H1-6-2) one day prior to grafting and daily after grafting for nine days. (Mean survival time [MST] of the free skin graft was 11.2 ± 0.6 days in the control group and 13.4 ± 0.3 days in the 1A29 + WT-1 treated group [p