Lanthanide chelates as new fluorochrome labels for cytochemistry

Anti-rabbit IgG labeled with a new fluorescent europium chelate was used to localize rabbit IgG to human smooth muscle myosin in a histological section. The antibody labeled with the europium chelate could be viewed with a conventional fluorescence microscope with a steady-state light source. This r...

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Veröffentlicht in:	The journal of histochemistry and cytochemistry 1988-11, Vol.36 (11), p.1449-1451
Hauptverfasser:	Soini, EJ, Pelliniemi, LJ, Hemmila, IA, Mukkala, VM, Kankare, JJ, Frojdman, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Fluorescent Antibody Technique Fluorescent Dyes Fundamental and applied biological sciences. Psychology General aspects Humans Immunohistochemistry Microscopy, Fluorescence Muscle, Smooth - analysis Myosins - analysis
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Anti-rabbit IgG labeled with a new fluorescent europium chelate was used to localize rabbit IgG to human smooth muscle myosin in a histological section. The antibody labeled with the europium chelate could be viewed with a conventional fluorescence microscope with a steady-state light source. This result encourages the development of a time-resolved fluorescence microscope, because a significant improvement in the signal-to-noise ratio can be anticipated.
ISSN:	0022-1554 1551-5044
DOI:	10.1177/36.11.3049792