Differences in the responses to purinergic nerve stimulation and applied ATP in the guinea-pig vas deferens
The responses to sympathetic nerve stimulation and to applied ATP in the guinea-pig vas deferens were compared. Nifedipine (10 μ) markedly reduced the non-adrenergic neural contraction but only partially blocked the contractions produced by bath-applied ATP. Suramin (300 μM) also markedly reduced th...
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| Veröffentlicht in: | Journal of the autonomic nervous system 1996-02, Vol.57 (1), p.93-100 |
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| container_title | Journal of the autonomic nervous system |
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| creator | Reilly, M.J. Hirst, G.D.S. |
| description | The responses to sympathetic nerve stimulation and to applied ATP in the guinea-pig vas deferens were compared. Nifedipine (10 μ) markedly reduced the non-adrenergic neural contraction but only partially blocked the contractions produced by bath-applied ATP. Suramin (300 μM) also markedly reduced the contractile responses produced by nerve stimulation, but had no significant effect on the contractions produced by bath-applied ATP. Using intracellular recording techniques, nerve stimulation was shown to produce an excitatory junction potential which was abolished by suramin (1 μM). Ionophoretic application of ATP and bath-applied ATP also produced a depolarization. Suramin (1 μ) failed to abolish the response to bath-applied ATP and enhanced the ionophoretically induced depolarization. These results suggest either that ATP is not a transmitter in the vas deferens or that two classes of purinoceptor are present, one suramin-sensitive receptor which produces a contraction via the opening of voltage-dependent Ca z+ channels, and another which is suramin-resistant and produces a contraction by another means. |
| doi_str_mv | 10.1016/0165-1838(95)00105-0 |
| format | Article |
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Nifedipine (10 μ) markedly reduced the non-adrenergic neural contraction but only partially blocked the contractions produced by bath-applied ATP. Suramin (300 μM) also markedly reduced the contractile responses produced by nerve stimulation, but had no significant effect on the contractions produced by bath-applied ATP. Using intracellular recording techniques, nerve stimulation was shown to produce an excitatory junction potential which was abolished by suramin (1 μM). Ionophoretic application of ATP and bath-applied ATP also produced a depolarization. Suramin (1 μ) failed to abolish the response to bath-applied ATP and enhanced the ionophoretically induced depolarization. These results suggest either that ATP is not a transmitter in the vas deferens or that two classes of purinoceptor are present, one suramin-sensitive receptor which produces a contraction via the opening of voltage-dependent Ca z+ channels, and another which is suramin-resistant and produces a contraction by another means.</description><identifier>ISSN: 0165-1838</identifier><identifier>EISSN: 1872-7476</identifier><identifier>DOI: 10.1016/0165-1838(95)00105-0</identifier><identifier>PMID: 8867091</identifier><identifier>CODEN: JASYDS</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adenosine Triphosphate - pharmacology ; Animals ; ATP ; Biological and medical sciences ; Dose-Response Relationship, Drug ; Electric Stimulation ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Guinea-pig vas deferens ; Male ; Mammalian male genital system ; Membrane Potentials - drug effects ; Morphology. Physiology ; Nifedipine - pharmacology ; P 2X-Purinoceptor ; Purinergic nerve ; Suramin ; Suramin - pharmacology ; Vas Deferens - drug effects ; Vertebrates: reproduction</subject><ispartof>Journal of the autonomic nervous system, 1996-02, Vol.57 (1), p.93-100</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-2a0948546ce6f2a5fc03e0f6b4dc4cee9c725e2d46071ed261294af8a8d9e02a3</citedby><cites>FETCH-LOGICAL-c512t-2a0948546ce6f2a5fc03e0f6b4dc4cee9c725e2d46071ed261294af8a8d9e02a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2970750$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8867091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reilly, M.J.</creatorcontrib><creatorcontrib>Hirst, G.D.S.</creatorcontrib><title>Differences in the responses to purinergic nerve stimulation and applied ATP in the guinea-pig vas deferens</title><title>Journal of the autonomic nervous system</title><addtitle>J Auton Nerv Syst</addtitle><description>The responses to sympathetic nerve stimulation and to applied ATP in the guinea-pig vas deferens were compared. Nifedipine (10 μ) markedly reduced the non-adrenergic neural contraction but only partially blocked the contractions produced by bath-applied ATP. Suramin (300 μM) also markedly reduced the contractile responses produced by nerve stimulation, but had no significant effect on the contractions produced by bath-applied ATP. Using intracellular recording techniques, nerve stimulation was shown to produce an excitatory junction potential which was abolished by suramin (1 μM). Ionophoretic application of ATP and bath-applied ATP also produced a depolarization. Suramin (1 μ) failed to abolish the response to bath-applied ATP and enhanced the ionophoretically induced depolarization. These results suggest either that ATP is not a transmitter in the vas deferens or that two classes of purinoceptor are present, one suramin-sensitive receptor which produces a contraction via the opening of voltage-dependent Ca z+ channels, and another which is suramin-resistant and produces a contraction by another means.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>ATP</subject><subject>Biological and medical sciences</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electric Stimulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Guinea-pig vas deferens</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Membrane Potentials - drug effects</subject><subject>Morphology. Physiology</subject><subject>Nifedipine - pharmacology</subject><subject>P 2X-Purinoceptor</subject><subject>Purinergic nerve</subject><subject>Suramin</subject><subject>Suramin - pharmacology</subject><subject>Vas Deferens - drug effects</subject><subject>Vertebrates: reproduction</subject><issn>0165-1838</issn><issn>1872-7476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EKtvCNwDJB4TKIcX22rF9QarKv0qVyqGcLdceL4ZsEjzJSnz7Ot1lj3CwxvL85o31HiGvOLvgjLfv61ENN2tzbtU7xjhTDXtCVtxo0Wip26dkdUSek1PEnxXSzJoTcmJMW298RX59zClBgT4A0tzT6QfQAjgOPdaHaaDjXHIPZZMDrWUHFKe8nTs_5aGnvo_Uj2OXIdLLu29_BTZzHfHNmDd055FGeNyAL8iz5DuEl4d6Rr5__nR39bW5uf1yfXV50wTFxdQIz6w0SrYB2iS8SoGtgaX2XsYgA4ANWigQUbZMc4ii5cJKn4w30QITfn1G3u51xzL8ngEnt80YoOt8D8OMThsp14az_4JcKWO15BWUezCUAbFAcmPJW1_-OM7cEoZbnHaL084q9xiGW_RfH_Tn-y3E49DB_dp_c-h7DL5Lxfch4xETVjOtFpkPewyqabsMxWHIS2IxFwiTi0P-9z8eAPK6phk</recordid><startdate>19960205</startdate><enddate>19960205</enddate><creator>Reilly, M.J.</creator><creator>Hirst, G.D.S.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19960205</creationdate><title>Differences in the responses to purinergic nerve stimulation and applied ATP in the guinea-pig vas deferens</title><author>Reilly, M.J. ; Hirst, G.D.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-2a0948546ce6f2a5fc03e0f6b4dc4cee9c725e2d46071ed261294af8a8d9e02a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>ATP</topic><topic>Biological and medical sciences</topic><topic>Dose-Response Relationship, Drug</topic><topic>Electric Stimulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Guinea-pig vas deferens</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Membrane Potentials - drug effects</topic><topic>Morphology. Physiology</topic><topic>Nifedipine - pharmacology</topic><topic>P 2X-Purinoceptor</topic><topic>Purinergic nerve</topic><topic>Suramin</topic><topic>Suramin - pharmacology</topic><topic>Vas Deferens - drug effects</topic><topic>Vertebrates: reproduction</topic><toplevel>online_resources</toplevel><creatorcontrib>Reilly, M.J.</creatorcontrib><creatorcontrib>Hirst, G.D.S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the autonomic nervous system</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reilly, M.J.</au><au>Hirst, G.D.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in the responses to purinergic nerve stimulation and applied ATP in the guinea-pig vas deferens</atitle><jtitle>Journal of the autonomic nervous system</jtitle><addtitle>J Auton Nerv Syst</addtitle><date>1996-02-05</date><risdate>1996</risdate><volume>57</volume><issue>1</issue><spage>93</spage><epage>100</epage><pages>93-100</pages><issn>0165-1838</issn><eissn>1872-7476</eissn><coden>JASYDS</coden><abstract>The responses to sympathetic nerve stimulation and to applied ATP in the guinea-pig vas deferens were compared. Nifedipine (10 μ) markedly reduced the non-adrenergic neural contraction but only partially blocked the contractions produced by bath-applied ATP. Suramin (300 μM) also markedly reduced the contractile responses produced by nerve stimulation, but had no significant effect on the contractions produced by bath-applied ATP. Using intracellular recording techniques, nerve stimulation was shown to produce an excitatory junction potential which was abolished by suramin (1 μM). Ionophoretic application of ATP and bath-applied ATP also produced a depolarization. Suramin (1 μ) failed to abolish the response to bath-applied ATP and enhanced the ionophoretically induced depolarization. These results suggest either that ATP is not a transmitter in the vas deferens or that two classes of purinoceptor are present, one suramin-sensitive receptor which produces a contraction via the opening of voltage-dependent Ca z+ channels, and another which is suramin-resistant and produces a contraction by another means.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8867091</pmid><doi>10.1016/0165-1838(95)00105-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of the autonomic nervous system, 1996-02, Vol.57 (1), p.93-100 |
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| language | eng |
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| subjects | Adenosine Triphosphate - pharmacology Animals ATP Biological and medical sciences Dose-Response Relationship, Drug Electric Stimulation Fundamental and applied biological sciences. Psychology Guinea Pigs Guinea-pig vas deferens Male Mammalian male genital system Membrane Potentials - drug effects Morphology. Physiology Nifedipine - pharmacology P 2X-Purinoceptor Purinergic nerve Suramin Suramin - pharmacology Vas Deferens - drug effects Vertebrates: reproduction |
| title | Differences in the responses to purinergic nerve stimulation and applied ATP in the guinea-pig vas deferens |
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