Transcriptional downregulation of stromelysin by tetracycline
We investigated the role of tetracycline in the transcriptional regulation of matrix metalloproteinases. Using interleukin‐1β (IL‐1) induced stromelysin as a model system, we describe the repression of the endogenous stromelysin RNA accumulation, as well as the transcriptional inhibition of various...
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Veröffentlicht in: | Journal of cellular biochemistry 1996-03, Vol.60 (3), p.341-347 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We investigated the role of tetracycline in the transcriptional regulation of matrix metalloproteinases. Using interleukin‐1β (IL‐1) induced stromelysin as a model system, we describe the repression of the endogenous stromelysin RNA accumulation, as well as the transcriptional inhibition of various stromelysin promoter/chloramphenicol‐acetyltransferase constructs in transient transfection assays. The inhibition occurred in a dose‐dependent fashion, with an IC50 of about 1 μM. Our results suggest that the transcriptional inhibition by tetracycline is not due to a block of activity of the activating protein complex 1 (AP‐1) but is mediated by sequences upstream of the AP‐1 binding site. © 1996 Wiley‐Liss, Inc. |
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ISSN: | 0730-2312 1097-4644 |
DOI: | 10.1002/(SICI)1097-4644(19960301)60:3<341::AID-JCB6>3.0.CO;2-W |