Localization of the gene for the human serotonin 5-HT(2B) receptor to chromosome 2

A 483 bp Bgl II/Sal I repeat free fragment was isolated from the vector containing the human serotonin 5-HT(2B) receptor. This fragment contained part of the coding sequence located within the last exon and the entire 3'-non-translated region. It was used to probe, by Southern blots, Pst I dige...

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Veröffentlicht in:	Molecular membrane biology 1996-01, Vol.13 (1), p.29-31
Hauptverfasser:	Horton, Y M, Lubbert, H, Houslay, M D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromosome Mapping Chromosomes, Human, Pair 2 Cricetinae Deoxyribonucleases, Type II Site-Specific - metabolism DNA Probes Humans In Situ Hybridization Mice Receptor, Serotonin, 5-HT2B Receptors, Serotonin - genetics Receptors, Serotonin - metabolism
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Zusammenfassung:	A 483 bp Bgl II/Sal I repeat free fragment was isolated from the vector containing the human serotonin 5-HT(2B) receptor. This fragment contained part of the coding sequence located within the last exon and the entire 3'-non-translated region. It was used to probe, by Southern blots, Pst I digested DNA from a series of 20 rodent-human somatic cell hybrid lines, each of which contained different complements of human chromosomes. This 5-HT(2B) receptor probe showed hybridization to a 2-7 kb Pst I fragment which revealed 100 percent concordance with location of the gene for this 5-HT receptor variant at chromosome 2.
ISSN:	0968-7688